基于代谢组学技术的杭麦冬和川麦冬药效差异性研究

Pharmacodynamic differences between Hangmaidong and Chuanmaidong based on metabonomics

运用超高效液相-四级杆飞行时间质谱(UPLC-Q-TOF/MS)结合多变量统计学分析,探讨利用代谢组学的研究方法比较杭麦冬和川麦冬对正常大鼠血浆内源性代谢物干预作用的差异性。结果显示川麦冬低、中剂量组与对照组相近,高剂量组则有明显区别;杭麦冬高、中、低剂量组与对照组明显不同,剂量越大距离越远;具有显著性差异的内源性代谢产物为溶血磷脂酰胆碱类物质,与心血管系统类疾病紧密相关。与川麦冬给药组相比,杭麦冬组对正常大鼠血浆内源性代谢产物的干预作用更显著。从药效学上看,杭麦冬和川麦冬差异显著。

To evaluate the differences of Ophiopogonjaponicus from different cultivations, the metabolomics based method was conducted to compare the effects of Hangmaidong and Chuanmaidong （Chinese name） on plasma endogenous metabolites of normal rats. Data were collected by ultra performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-Q-TOF/MS）, and were analyzed by multivariate statistical method, such as Principal Component Analysis and Orthogonal Signal Correction Partial Least Square Discriminant Analysis. Results revealed that the plasma metabolites profiling of low and middle dose group of Chuanmaidong were similar to the control group, but different from the high dose group obviously. Meanwhile the high, middle and low dose groups of Hangrnaidong were different from control group notably, and the difference is dose dependent. Lysophosphatidylcholines, the possible endogenous metabolites which contribute to the classification most significantly, are closely related to cardiovascular system diseases. Compared with the group of Chuanmaidong, Hangmaidong has greater impact on the plasma metabolic profiling of normal rats. Hangmaidong and Chuanmaidong showed significant differences pharmacodynamically.