骨转录因子Runx2和骨桥蛋白OPN在人乳腺癌组织中的表达及其与乳腺癌微钙化的关系

The expressions of Runx2 and OPN protein in human breast cancer tissue and their association with microcalcification

目的观察骨转录因子Runx2、骨桥蛋白(osteopontin,OPN)在人乳腺癌组织中的表达,并进一步探讨其与乳腺癌微钙化形成间的关系。方法利用免疫组化方法观察骨转录因子Runx2和骨桥蛋白OPN在62例乳腺癌组织中的表达。根据乳腺癌患者钼靶X线片中微钙化的有无与数量多少,将62例乳腺癌分为3组,即无钙化组、少量钙化组以及大量钙化组,观察Runx2和OPN蛋白表达与不同乳腺癌微钙化之间的关系。结果 Runx2蛋白在人类乳腺癌组织中表达阳性率为72.6%(45/62)。OPN蛋白在人类乳腺癌组织中表达阳性率为79.0%(49/62)。Runx2和OPN蛋白的阳性表达与乳腺癌钼靶X线片中微钙化的出现和数量密切相关,随着微钙化从无到有以及由少至多,Runx2(χ2=15.686,P<0.05)和OPN(χ2=16.161,P<0.05)蛋白的阳性表达率呈逐步增高的趋势。但Runx2和OPN阳性表达与乳腺癌钼靶片中微钙化形态无关。结论 Runx2和OPN蛋白在人类乳腺癌组织中高表达,其可能参与了乳腺癌微钙化的形成过程。

Objective To explore the expressions of Runx2 and osteopontin （OPN） in human breast cancer tissue and their association with the formation of microcalcification. Methods The expressions of Runx2 and OPN were detected in breast cancer tissue of 62 cases by immunohistochemical method. Then the 62 cases were divided into three groups： non-microcalcification, mild-microcalcification and high-microcalcification groups according to the amount of microcalcification shown on mammography in breast cancer. The association of Runx2 and OPN expressions with microcalcification was further studied. Results The positive expression rate of Runx2 and OPN in human breast cancer tissue was 72.6% and 79.0%, respectively. The positive expression of Runx2 and OPN proteins was significantly correlated with the amount of microcalcification, but not with the morphology of microcalcification in breast cancer tissue. The positive rate of Runx2 and OPN was gradually elevated with the increase in the amount of microcalcification （ X^2 = 15. 686, P〈0.05 ; X^2 = 16. 161, P〈0.05）, respectively. Conclusion Runx2 and OPN proteins are highly expressed in human breast cancer tissue and they may participate in the process of microcalcification formation in breast cancer.