青黛对白假丝酵母菌抗菌作用的研究

Determination of Minimum Bactericidal Concentration of Indigo Naturalis Minimal Inhibitory Concentrations(mic) on White Candida

目的:研究青黛对白假丝酵母菌抑菌与杀菌作用。方法:运用琼脂扩散法、试管稀释法测定青黛悬浊液对于白假丝酵母菌的抑菌环直径、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:青黛悬浊液1∶8、1∶16、1∶32、1∶64稀释液对白假丝酵母菌抑菌环直径分别为(27.22±5.22)mm、(34.97±3.83)mm、(31.26±5.74)mm、(24.95±5.12)mm;青黛悬浊液1∶16稀释液的抑菌环直径明显大于其他浓度稀释液的抑菌环直径(P<0.01)。青黛悬浊液对白假丝酵母菌MIC为62.5 mg/m L,MBC为62.5 mg/m L。结论:青黛悬浊液对白假丝酵母菌有较明显的的抑制与杀菌作用。

Objective： To study the ＂Qingdai （Indigo Naturalis）＂ bacteriostatic and bactericidal action on White Candida. Methods： AGAR diffusion method and tube dilution method were applied to determine the ＂Qingdai （Indigo Naturalis）＂ suspensions for white candida bacteriostatic ring diameter, minimum baeteriostatic concentration （MIC） and minimum bactericidal concentration （MBC）. Results： The bacteriostatie ring diameter 4 diluent eandida dialogue of ＂Qingdai （Indigo Naturalis）＂ suspension 1：8, 1：16, 1：32 and 1：6 are respectively（27.22±27.22）mm, （34.97-3.83） mm, （31.26-5.74） mm and mm （24.95＋5.12）mm; ＂Qingdai （Indigo Naturalis）＂ suspension 1：16 diluent concentration of bacteriostatic ring diameter significantly greater than other diluent of bacteriostatic ring diameter（P〈0.01）. ＂Qingdai （Indigo Naturalis）＂ suspension dialogue candida MIC is 62.5 mg/mL, MBC is 62.5mg/mL Conclusion： ＂Qingdai （Indigo Naturalis）＂ suspension dialogue candida has the obvious inhibitory and bactericidal action.