高效液相色谱法测定复方生地合剂中绿原酸的含量

Determination of Chlorogenic Acid in Fufang Shengdi Mixture by HPLC

目的：建立复方生地合剂中绿原酸含量测定的高效液相色谱法。方法色谱柱（250 mm±4.6 mm，5μm），流动相：乙腈-0.4%磷酸溶液（10：90），流速1 mL·min-1，柱温30℃，检测波长327 nm。结果绿原酸在0.0624-4.68μg范围内线性关系良好。R=0.9999，平均回收率为100.7%，RSD=1.7%（n=6）。结论该方法操作简便，重复性高，可用于复方生地合剂的质量控制。

Objective To establish a HPLC method for the determination of chlorogenic acid in Fufang Shengdi mixture. Methods A SHISEIDO MG C18 column（250 mm±4.6 mm, 5μm） was used with the mobile phase of 0.4%phosphoric acid and acetonitrile for isocratic elution （10：90） at the wavelength of 327 nm, with the flow rate of 1mL·min-1 and the column temperature was 30℃. Results The calibration curves for chlorogenic acid were linear in the concentration ranges of 0.0624-4.68μg, and the correlation coefficient was 0.999999. The average recovery was 100.7%,（RSD=1.7%, n=6）. Conclusion This method is simple and reproducible,and is suitable for the quality control of the Fufang Shengdi mixture.