建兰新品种黄金小神童组培育苗集成技术的优化

Optimization of Integrated Technology for Tissue Culture of Cymbidium Golden Elf Sundust

为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明：侧芽茎尖在消毒前先用2％维生素水溶液浸泡30 min后再用0.1％升汞常规消毒,能有效延缓外植体褐化时间和保持外植体活力,外植体褐化率在15％以下;1/2 MS＋1％琼脂＋1％蔗糖＋1 g/LAC＋1 g/L Na2SO3为筛选出的各阶段通用最佳基本培养基;最佳培养条件为温度25～28℃,光照时间14 h/d,光照强度30～40μmol/（m2·s）;最佳激素处理,初诱导为TDZ 3.0 mg/L ＋NAA 0.2 mg/L;继代增殖为TDZ3.5 mg/L＋NAA 0.16 mg/L;生根诱导为6-BA 0.3 mg,/L＋ NAA 1.2 mg/L.生根培养70～80d,大棚炼苗15～20d后出瓶移栽,用蛭石做基质覆盖水藓的移栽方法较单独用粉碎树皮或水藓的移栽效果好,移栽成活率达90％以上.

The integrated technology for tissue culture of Cymbidium Golden Elf Sundust was optimized by screening optimum culture conditions for resistance to browning of explants,induced differentiation of explants,protocorm subculture multiplication and root induction of cluster buds to provide a technological reference for industrialized seedling production of Cymbidium Golden Elf Sundust.The results showed that：（i） The apexes of lateral buds soaked with 2 ％ Vc solution for 30min before regular disinfection of 0.1％ corrosive sublimate can effectively delay browning time of explants and keep explants vigor,and the explant browming rate is below 15 ％ ; （ii） The optimum basic medium for different culture stages is 1/2 MS ＋ 1％ agar ＋ 1％ sucrose ＋ 1 g/L AC ＋ 1 g/L Na2 SO3 ; （iii） The optimum culture condition is at 25-28 ℃ for 14 h/d lightng time under 30-40 pmol/（m2 · s） light intensity; （iv） The optimum hormone treatment for initial induclion,subculture multiplication and root induction is 3.0 mg/L TDZ ＋0.2 mg/L NAA,3.5 mg/L TDZ ＋0.16 mg/L NAA and 0.3 mg/L 6-BA ＋ 1.2 mg/L NAA respectively.The seedlings sfter 70-80 d of rooting culture and 15-20 d of hardening-seedling in a greenhouse can be transplanted in pots vermiculite covered with sphagnum and its transplant survival rate reaches above 90 ％.