离焦诱导豚鼠近视眼视网膜超微结构及凋亡研究

Retinal apoptosis and ultrastructural features of defocus- induced myopia in guinea pigs

目的:观察离焦诱导豚鼠近视眼视网膜超微结构特征及凋亡情况。方法:将30只3周龄的三色豚鼠用-10.00D硬性角膜接触镜(rigid gas permeable contact lens,RGP)对右眼进行离焦诱导,左眼不戴镜。3wk后散瞳检影观察屈光度变化;电子透射显微镜观察视网膜超微结构特征;TUNEL免疫荧光染色检测视网膜细胞凋亡情况。结果:右眼诱导前后屈光度差值为3.28±0.21D,左眼为1.55±0.23D,二者比较差异有显著统计学意义(P<0.01);电镜结果显示,3wk后诱导眼视网膜可见膜盘结构的水肿,部分脱落;线粒体肿胀变形,部分空泡化;内外核层细胞膜不规则收缩,核染色质不规则聚集等凋亡特征;TUNEL免疫荧光染色显示诱导眼视网膜细胞凋亡发生率为(2.42±1.24)%,对照眼为(0.29±0.08)%,二者比较差异有显著统计学意义(P<0.01)。结论:在离焦诱导的豚鼠近视眼的形成过程中伴随着视网膜细胞的异常凋亡。

AIM： To observe retinal ultrastructural features and retinal cell apoptosis of defocus induced myopia in guinea pigs. METHODS： Thirty guinea pigs （3- wk old） were received -10.00D rigid contact lenses （RGP） on the right eye and without wearing glasses on the left eye. Diopter change with cycloplegic retinoscopy, ultrastructural features of the retina with transmission electron microscopy were observed, the apoptosis of retinal cell by TUNEL immunofluorescence staining after 3wk were detected. RESULTS： Eye diopter difference was 3.28±0.21D in the right eye and 1.55±0.23D in the left eye before and after induction. There was significantly statistical difference （ P〈0.01 ） ; Retinal ultrastructural features were showed with transmission electron microscopy （TEM） the retinal disk membranes edema, some shedding, mitochondrial swelling and deformation, small amount of vacuolar changes in the inner and outer nuclear layer cells. Some characteristics of apoptosis were also be seen such as cell membrane irregular contractions, irregular nuclear chromatin aggregation. The incidenceof retinal cell apoptosis was （2.42±1.24）% in the right eye and （0.29±0.08）% in the left eye after induction. There was significantly statistical difference （ P〈0.01 ）. CONCLUSION ： Accompanied by abnormal apoptosis of retinal cells during the formation of defocus-induced myopia in guinea pigs.