Beckman AU5800生化分析仪交叉污染试验设计与应用

Design and application of cross contamination experiment for Beckman AU5800 automatic biochemical analyzer

目的设计并验证适合Beckman AU5800全自动生化分析仪试剂针和搅拌棒的交叉污染分析方法。方法以反应盘外圈项目（AST、ALP、TP、LDH、CK-MB、HBDH、Urea、UA、TG、LDL-C、APOB、Mg、P、Zn、AMY、CHE、LPS、ADA）作为试验项目,按设计的检测顺序进行交叉污染试验,各项目间的影响率以不超过95%-105%为判断标准。结果全部外圈项目中TP、HBDH、APOB、TG分别对CK-MB、Mg、Mg、LPS存在交叉污染。结论通过建立的方法能有效、快速地对Beckman AU5800全自动生化分析仪上的所有项目进行交叉污染试验,初步确定交叉污染产生的关联项目并采取相应的污染避免措施减少交叉污染,确保检验结果的准确。

Objective To design and verify an analytical method for the cross contamination of Beckman AU5800 automatic biochemical analyzer′s reagent needle and stir bar.Methods The outer ring items（AST,ALP,TP,LDH,CK-MB,HBDH,Urea,UA,TG,LDL-C,APOB,Mg,P,Zn,AMY,CHE,LPS,ADA）were tested as the experiment objects.The cross contamination experiment was proceeded according to the test order.The impact rate range of 95%-105% was the criteria for cross contamination of items.Results Among all of the outer ring items,TP,HBDH,APOB and TG were found to have cross contamination effect on CK-MB,Mg,Mg and LPS respectively.Conclusion The method established in this experiment can effectively analyze the cross contamination of all the items in Beckman AU5800 automatic biochemical analyzer.In addition,it can help to adopt corresponding measures to reduce cross contamination and assure the accuracy of test results.