荧光定量聚合酶链反应用于非脊髓灰质炎肠道病毒定性方法的评估

Qualitative Assessment of Non-polio Enterovirus With Fluorescence Quantitative Polymerase Chain Reaction Methods

目的在河北省脊灰实验室应用荧光定量聚合酶链反应(PCR)法对非脊髓灰质炎肠道病毒(NPEV)进行定性。方法采用荧光定量PCR法对既往河北省脊灰实验室分离到NPEV进行检测,并将检测结果与中和实验鉴定结果和新流程方法检测结果进行比较分析。结果 75株用中和实验方法定性的NPEV与荧光定量PCR方法检测的符合率为98.7%(74/75),33株用新检测流程定性的NPEV与荧光定量PCR方法检测的符合率为81.8%。结论荧光定量PCR用于NPEV进行定性的检测方法是可行的。

Objective To qualitatively determine the non-polio enterovirus （NPEV） with fluorescence quantitative polymerase chain reaction （PCR） in the Polio Lab of Hebei Province. Methods Fluorescent quantitative PCR method was adopted to detect the previous isolated NPEV, and the detection results were compared with the that of neutralization test and new process experiment. Results Seventy five strains determined by PCR were confirmed with the neutralization test, with a coincidence rate of 98. 7%; 33 strains determined by PCR were confirmed with the results of new process experiment, with a coincidence rate of 81.8 %. Conclusion Fluorogenic quantitative PCR is qualified for detection of NPEV.