摘要
据报道,拟南芥一氧化氮(nitric oxide,NO)合成缺陷突变体noa1较野生型Col-0抗旱,而个别抗旱相关基因表达的增强是其抗旱机理之一.为了证实该结果及研究NO信号分子在抗旱中的作用,系统地对8种共12个不同类型的抗旱相关基因在干旱处理不同时间的拟南芥野生型和noa1及亚硝基谷胱甘肽还原酶功能缺失突变体gsnor1-3间的表达情况进行了反转录PCR分析.研究结果表明,抗旱相关基因的表达在拟南芥不同基因型间无明显差异,noa1的耐旱性与抗旱相关基因的表达无显著相关性.
About nitric oxide（ NO）,a small gaseous molecule that regulated many biological processes in plants,was reported that Arabidopsis noa1 mutant,defective in NO production,was more resistant to water deficit than wilde-type Col-0 and the higher expression levels of a few drought-related genes were correlated with the enhanced drought tolerance of noa1. To confirm the results and further investigate the role of NO in drought tolerance,the plants of Col-0,noa1 as well as gsnor1-3,a GSNOR1 loss-of-function mutant,defective in de-nitrosylation were subjected to drought treatment for various length of time and RT-PCR analysis was performed for total of 12 drought-related genes that belonged to 8 different gene families. The RT-PCR analysis indicated that no significant differences were observed for the expression of genes related to drought resistance /tolerance among Col-0,noa1 and gsnor1-3 and the drought tolerance displayed by noa1 might not result from higher expression of genes involved in drought tolerance.
出处
《浙江师范大学学报（自然科学版）》
CAS
2014年第4期367-372,共6页
Journal of Zhejiang Normal University:Natural Sciences
基金
国家自然科学基金资助项目(31371407)
浙江省钱江人才计划项目(2013R10074)
人力资源和社会保障部留学人员择优资助项目
浙江省自然科学基金资助项目(LY12C14001)
