干扰素释放酶联免疫法（TB-IGRA）与蛋白芯片法检测结核分枝杆菌的方法比较

Comparison of TB-IGRA and Protein Chip in the Diagnosis of Mycobacterium Tuberculosis

目的：比较干扰素释放酶联免疫法（TB-IGRA）与蛋白芯片两种方法检测结核分枝杆菌的特异度、敏感度，评价其在结核病诊断中的价值。方法选取2013年10月-2014年3月收集共计78例临床诊断结核病及正常人外周血标本，分别采用TB-IGRA试剂盒和结核分枝杆菌 IgG抗体检测试剂盒（蛋白芯片）进行检测，并对检测结果进行分析比较。结果蛋白芯片法、TB-IGRA法检出结核分枝杆菌的灵敏度分别为34.5％及89.7％，差异有统计学意义（χ2＝26.95，P＜0.05）；二者的特异度分别为90.0％及95.0％，差异无统计学意义（χ2＝1.64，P＞0.05）。TB-IGRA法在肺结核和肺外结核患者阳性率分别为90.5％，89.2％，均高于蛋白芯片法（芯片法在肺结核和肺外结核患者阳性率分别为42.9％，29.7％）。结论 TB-IGRA法与蛋白芯片法比较，无论是诊断肺结核或肺外结核均具有阳性率高、敏感度较高的优点，可广泛用于结核病的早期筛查。

Objective To evaluate the advantages of TB-IGRA and protein chip to detect the Mycobacterium tuberculosis. Methods From October 2013 to March 2014,collected 78 cases of clinical diagnosis of tuberculosis and normal control’s pe-ripheral blood specimens,used TB-IGRA kits and Mycobacteriumtuberculosis IgG kit（protein chip）to detected respectively. The results were analyzed and compared.Results The sensitivity of protein chip and TB-IGRA in the detection of Mycobac-teriumtuberculosis were 34.5% and 89.7% respectively,which was statistically significant （χ2=26.95,P〈0.05）.The spe-cificity of protein chip and TB-IGRA were 90.0%,95.0% respectively,which were not statistically significant （χ2=1.64,P〉 0.05）.The positive rate of TB-IGRA and Protein chip in tuberculosis were 90.5% and 42.9%.The positive rate of TB-IGRA and Protein chipin extrapulmonary tuberculosis were 89.20% and 29.7% respectively.Conclusion Compared TB-IGRA and protein chip,either diagnose tuberculosis or extrapulmonary tuberculosis has highly positive rate and sensitivity, TB-IGRA can be widely used in the early screening of tuberculosis.