大孔树脂纯化废酒花黄腐酚及其抗DNA氧化损伤的活性

Purification of xanthohumol by macroporous resin from humulus lupulus and its inhibition activity against DNA oxidative damage

采用大孔树脂从酿造废酒花中分离纯化黄腐酚,并且检测了黄腐酚抗DNA氧化损伤的活性。结果表明:HPD100A树脂对黄腐酚的吸附率为100%,解吸率为77.69%,适合于废酒花黄腐酚的纯化。最佳工艺条件为:上样液黄腐酚质量浓度0.25mg/m L、上样量15BV、上样液p H5.5、上样流速2BV/h,洗脱剂为95%乙醇,洗脱流速2BV/h,洗脱剂用量3BV。在此条件下,可将黄腐酚纯度由纯化前的4.95%提高为51.50%,回收率为72.56%。结果表明,分离得到的黄腐酚纯化物对Phen-Cu SO4-VC诱导DNA氧化损伤的抑制作用(EC50为0.22mg/m L)显著优于抗坏血酸(EC50为0.51mg/m L)和芦丁(EC50为0.93mg/m L),具有显著地抗DNA氧化损伤活性。

Xanthohumol was separated and purified by macroporous adsorption resin from waste hops （Humulus lupulus L.）,and its inhibition activity against DNA oxidative damage was investigated. The resulted showed that HPD100A was appropriated to purify xanthohumol from waste hops,with adsorption rate was 100% and desorption rate was 77.69%. The optimal conditions for purification were as follows ：the loading concentration of xanthohumol was 0.25mg/mL,the loading volume was 15BV,the pH of loading solution was 5.5,the loading rate was 2BWh,the elution was 95% ethanol,the elution rate was 2BWh and the elution volume was 3BV. Under the optimized conditions,the purity of xanthohumol in extract was increased from 4.95% to 51.50% ,with the recover rate was 72.56%. The purified xanthohumol was evaluated its inhibition activity against DNA oxidative damage. The result showed that the purified xanthohumol had dramatically inhibition activity against DNA oxidative damage induced by Phen-CuSO4-Vc,and its inhibition activity（EC50=0.22mg/mL） significantly better than that of ascorbic acid（EC50=0.51mg/mL） and rutin（EC50=0.93mg/mL）.