彝族乳腺癌遗传易感性CYP1A1基因MspI多态性的相关性研究

Relationship between MspIpolymorphism of CYP1A1 gene and susceptibility to breast cancer of Yi nationality in Yunnan

目的探讨云南省彝族乳腺癌遗传易感性与CYP1A1基因MspⅠ位点多态性的关系。方法采用聚合酶链反应-限制性片段长度多态性PCR-RFLP技术,对云南省肿瘤医院51例彝族乳腺癌患者及60例健康女性人群的CYP1A1基因3′端限制性内切酶MspI位点基因多态性进行分析。结果乳腺癌组C等位基因分布频率(51.0%)明显高于对照组(33.3%),差异均有统计学意义(均P<0.05)。3种基因型分布频率与对照组相比,差异有统计学意义(均P<0.05)。TC、CC基因型患乳腺癌的危险度分析OR(95%CI)分别是TT基因型的1.19和1.95倍。结论云南省彝族乳腺癌发病率可能与CYP1A1基因型有关,基因突变增加了患乳腺癌的风险。

Objective To study the relationship between Msp I Polymorphism of CYP1A1 gene and susceptibility to breast cancer in Yi nationality in Yunnan. Methods The polymorphism of restriction enzyme of 3＇-terminal of CYP1A1 was detected with PCR-RFLP technology. 60 healthy female people and 51 patients with breast cancer were examined by allele specific amplification. Results The distribution of CYP1A1-Msp I genotypes was statistically significantly different between breast cancer patients and control group（P〈0.05）. The allele C had higher frequency in patients with breast cancer than in control group（P〈0.05）. Significant differences of frequencies were found in genotype TT, TC and CC between the patients with breast cancer and the controls （P〈0.05）. The risks of TC and CC to breast cancer were 1.19 and 1.95 folds respectively to TT genotype. Conclusion Gene polymorphism of CYP1A1 genotypes may be associated with the risk of the breast cancer in Yi nationality in Yunnan. The mutation of CYP1A1 gene may increase the incidence of breast cancer.