金荞麦提取物对慢性阻塞性肺病大鼠血清细胞因子及肺组织病理形态学的影响

Effect of Extract of Fagopyrum Dibotrys on Serum Cytokines and Pulmonary Histopathological Changes in Chronic Obstructive Pulmonary Disease Rats

目的观察金荞麦提取物对慢性阻塞性肺病(COPD)大鼠模型的作用及机制。方法水提和醇提法制备金荞麦提取物;改良烟熏法复制SD大鼠COPD模型,实验分为正常组,模型组,金荞麦水提取物低、高剂量组,金荞麦醇提取物低、高剂量组。药物干预后,放射免疫法测定血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-8(IL-8)、Ⅲ型前胶原肽(PⅢ)、转化生长因子(TGF-β1)含量的变化,光镜观察肺组织病理形态学。结果模型组大鼠血清TNF-α、IL-6、IL-8、PⅢ、TGF-β1含量较正常组明显升高(P<0.01),肺组织病理损伤明显;金荞麦提取物可显著降低上述异常升高的指标(P<0.05)、改善肺组织病理损伤,其中金荞麦醇提物高剂量组作用最明显(P<0.01)。结论金荞麦提取物能抑制COPD大鼠的炎症反应,改善肺组织的病理损伤。

Objective To study the effect of the extract of Fagop yrum dibotrys（FD）on chronic obstructive pulmonary disease（COPD） rats and to explore the therapeutic mechanism for COPD. Methods FD water-extract and ethanolextract of were prepared. COPD in SD rats was induced by modified smoking method. SD rats were randomly divided into normal group, model group, low- and high-dose FD water-extraction groups, low- and high-dose FD ethanol-extraction groups. After drug intervention, the serum levels of tumor necrosis factor alpha（TNF-α）,interleukin 6（IL-6）,IL-8,precollagen peptide III（PIII）,transforming growth factor beta 1（TGF-β1）were detected by radioimmunoassay method. Histopathological changes of lung were also observed. Results Compared with the normal group, the serum levels of TNF-a, IL-6, IL-8, PIII, and TGF-β1 in the model group were increased significantly（P〈0.01）. The lung pathological injury was obvious in the model group. FD extract significantly decreased the abnormal rising of the above indexes, and improved the lung histopathological changes in rats, especially in high-dose FD ethanol-extraction group（P〈0.01）. Conclusion FD extract can inhibit the inflammatory response in COPD rats,and improve the lung histopathological changes.