苦参碱联合顺铂对宫颈癌SiHa细胞株中TSLC1基因表达的影响研究

Study on the effect of sophocarpidine combined with cisplatin on TSLC1 gene expression in cervical cancer Si Ha cells

目的:探究苦参碱联合顺铂对宫颈癌Si Ha细胞株、肺癌肿瘤抑制因子1(TSLC1)基因的表达及Si Ha细胞株对顺铂敏感性的影响,为宫颈癌的临床治疗提供根据。方法:采取四甲基偶氮唑蓝(MTT)法测定不同浓度苦参碱组、顺铂组以及联合组干预Si Ha细胞株48 h后的抑制率;采取实时荧光定量PCR(FQ-PCR)技术检测TSLC1 mRNA表达程度的变化。结果:苦参碱及顺铂对Si Ha细胞株的抑制率均呈剂量依赖关系,随着各组浓度的增高,TSLC 1mRNA的表达上调(P<0.05),不同浓度联合组对Si Ha细胞株的抑制率分别为40.13%、62.88%、67.35%、71.33%和75.11%,TSLC1 mRNA的表达量分别为8.61±0.02、14.29±0.01、27.52±0.06、62.19±0.01和93.12±0.01,与不同浓度苦参碱组及不同浓度顺铂组比较,差异均有统计学意义(P<0.05)。结论:苦参碱联合顺铂可抑制宫颈癌Si Ha细胞株的生成和发展,此机制可能和TSLC1 mRNA的表达上调有关,且苦参碱可以增强Si Ha细胞株对顺铂的敏感性。

Objective： To explore the effect of sophocarpidine combined with cisplatin on cervical cancer SiHa cells, tumor sup- pressor in lung cancer 1 （TSLC1） gene expression and the sensitivity of cervical cancer SiHa cells to cisplatin, provide a basis for clinical treatment of cervical cancer. Methods ： MTT method was used to detect the inhibition rates of cervical cancer SiHa cells after 45 hours in so- phocarpidine group （different concentrations）, cisplatin group （different concentrations） and combined treatment group （different concentra- tions ） ; real -time fluorescent quantitative PCR （FQ -PCR） was used to detect the change of TSLC1 mRNA expression. Results： The inhi- bition rates of sophocarpidine and cisplatin on cervical cancer SiHa cells showed dose - dependent manners, the expression of TSLC 1 mRNA was upregulated with the increase of concentration （ P 〈 0. 05 ） , the inhibition rates on cervical cancer SiHa cells in combined treatment group of different concentrations were 40. 13% , 62. 88% , 67.35% , 71.33% and 75.11%, respectively; the expression levels of TSLC1 mRNA were （8. 61 ±0.02）, （ 14. 29 ±0. 01 ）, （27.52 ±0. 06）, （62. 19 ±0.01 ） and （93.12 ±0. 01 ）, respectively, compared with sophocarpi- dine group and cisplatin group of different concentrations, there were statistically significant differences （ P 〈 0. 05 ） . Conclusion ： Sopho- carpidine combined with cisplatin can inhibit the growth and development of cervical cancer SiHa cells, which may be correlated with up - regulation of TSLC1 mRNA, sophocarpidine can enhance the sensitivity of cervical cancer SiHa cells to cisplatin.