RP-HPLC法同时测定明目颗粒中淫羊藿苷和仙茅苷含量

Determination of Icariin and Curculigoside in Mingmu Granules by RP-HPLC

目的:采用反相高效液相色谱法同时测定明目颗粒中淫羊藿苷和仙茅苷的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-1.2%冰乙酸(B)为流动相进行梯度洗脱。梯度洗脱程序为:0~10 min,8%~15%A;10~15 min,15%~25%A;15~25 min,25%~30%A;25 min^50 min,30%A。检测波长为283 nm,柱温30℃,流速1.0 m L/min,进样量:10μL。结果:淫羊藿苷在进样量为0.02~0.18μg范围内线性关系良好,Y=0.595X+1.17,r=0.999 5;仙茅苷在进样量为0.04~0.36μg范围内线性关系良好,Y=0.614X+2.737,r=0.999 5。淫羊藿苷和仙茅苷平均加样回收率符合含量测定的要求。结论:该方法准确、简单、灵敏,可用于明目颗粒中淫羊藿苷和仙茅苷的含量测定。

Objective ：To establish an RP-HPLC method for determining the content of Icariin and curculigoside in Mingmu Granules by RP-HPLC. Methods：A Cls column （4.6 mmx250 mm,5 bL m ）was used with a mobile phase of methanol （ A ） -1.2% acetic acid （ B ） at gradient elution. The gradient elution program was as follows：0-10 min,8%-15% A; 10-15 rain,15%-25% A; 15-25 rain,25%-30% A;25 min -50 min, 30% A. The detection was carried out at 283 nm. The column temperature was set at 30℃. The flow rate was kept at 1.0 mL/min and the sample volume was 10 μ L. Results ： The standard cure of Icariin was linear in the range of 0.02-0.18μ g Y=0.595X＋l.17 （r=0.999 5, n=5 ）. The standard cure of eurculigoside was linear in the range of 0.04-0.36 μg, Y=0.614X＋2.737 （ r=0.999 5, n=5 ）. Conclusion ： The method is simple, accurate, specific and sensitive for the determination of Ieariin and eurculigoside in Mingmu Granules.