高效液相色谱法测定不同来源岩黄连中不同部位脱氢卡维丁含量

Determination of Dehydrocavidine in Different Parts of Corydalis Saxicola Bunting from Various Origins by HPLC

目的 建立岩黄连中有效成分脱氢卡维丁的高效液相色谱（HPLC）含量测定方法,并对2种不同来源药材的不同部位进行分析,为岩黄连质量标准的制定提供参考.方法 采用SHISEIDO-SPOLAR C18（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈0.05 moL·L^-1磷酸二氢钠溶液（25∶75）,检测波长347 nm,流速1.0 mL·min^-1,柱温：30℃.结果 脱氢卡维丁进样量在0.024 1～0.482 0 μg范围内与峰面积线性关系良好（r=0.999 9）;平均回收率99.48％（RSD=1.44％,n=6）.不同生长方式的岩黄连脱氢卡维丁的含量差异较大,以野生含量较高;药材不同部位中以根部含量最高.结论 建立的脱氢卡维丁含量测定方法简便,准确,重复性好,可用于岩黄连中类似生物碱成分的质量控制.

Objective To establish a quantification method for the determination of dehydrocavidine in Corydalis saxicola Bunting by reversed phase high performance liquid chromatography （RP-HPLC）. Different parts of the herb from two different sources were analyzed in order to provide evidence for setting quality standards for Corydalis saxicola Bunting. Methods The samples were separated by RP-HPLC on a SHISEIDO-SPOLAR C l8 column（250 mm×4.6 mm,5μm） , with the mixture of acetonitrile and buffer solution of 0.05 moL·L^-1 sodium dihydrogen phosphate （ 25 ： 75 ） as the mobile phase. The detection wavelength was set at 347 nm, and the flowing rate was 1.0 mL · min^-1. The column temperature was kept at 30 ℃. Rosults The linear range of dehydrocavidine was within 0. 024 1-0. 482 0 μg （r = 0. 999 9）, and the average recovery was 99.48% with RSD of 1.44% （ n = 6 ）. There was great variation in the content of dehydrocavidine under different growing conditions. Wildly grown Corydalis saxicola Bunting had the highest content of dehydrocavidine, and the roots have the highest content among different parts of the plant. Conclusion The established method is simple, accurate, and reproducible, therefore can be applied to the quality control for analogous alkaloids in Corydalis saxicola Bunting.