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羧甲基壳聚糖温敏凝胶对NIH3T3细胞在病变牙根面附着和增殖的影响 被引量:2

Effects of carboxymethyl chitosan thermosensitive hydrogel on attachment and proliferation of NIH3T3 cells on the root surface of teeth with periodontitis
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摘要 目的:观察羧甲基壳聚糖(CMCS)温敏凝胶对病变牙根面NIH3T3细胞附着和增殖的影响。方法:60颗患牙制成60个牙根片,分为5组。A组:超声刮治+EDTA处理3 min+CMCS浸提液涂擦牙根表面5 min;B组:超声刮治+EDTA处理3 min+无菌生理盐水涂擦牙根表面5 min;C组:手动刮治+EDTA处理3 min+CMCS浸提液涂擦牙根表面5 min;D组:手动刮治+EDTA处理3 min+无菌生理盐水涂擦牙根表面5 min;E组(对照组):EDTA处理3 min+无菌生理盐水涂擦牙根表面5 min。将NIH3T3细胞与5组牙根片分别共培养16、24、72 h,采用细胞计数法和MTT法观察结果。结果:培养16 h时,A、B、C、D组细胞计数均高于对照组。培养24、72 h时,A、B、C、D组细胞增殖能力均高于对照组,且使用CMCS浸提液的A组及C组的细胞增殖能力高于使用无菌生理盐水的B组及D组(F=31.813和41.579,P<0.05)。结论:采用手动或超声进行牙周刮治及根面平整均有助于NIH3T3细胞的附着;联合使用CMCS温敏凝胶处理牙根表面可以促进NIH3T3细胞在牙根面的附着与增殖。 Aim:To observe the effect of carboxymethyl chitosan ( CMCS) thermosensitive hydrogel on the attachment and proliferation of NIH3T3 cells on the root slices with periodontitis after scaling and root planning (SRP).Methods:Sixty teeth with periodontitis were used to prepare root slices and allocated into 5 groups randomly .Group A and group B were trea-ted by SRP using ultrasonator ,and group C and group D through manual treatment .After sterilizing the slices ,all of them were treated by EDTA for 3 min,then group A and group C were smeared with CMCS thermosensitive hydrogel ,and the other 3 groups were smeared by normal saline for 5 min.Then the NIH3T3 cells and the root slices were cocultured for 16,24,and 72 h,and the results were observed by cell counting method or MTT assay .Results:At 16 h,there were more cells in groups A,B, C,and D than those in control.At 24 and 72 h,the OD values of groups A,B,C,and D were higher than those in control;the OD values of groups A and C using CMCS thermosensitive hydrogel were higher than those in groups B and D using normal saline (F=31.813 and 41.579,P〈0.05).Conclusion:Treating the teeth with SRP using ultrasonator or by hand both help the attach-ment of NIH3T3 cells.CMCS thermosensitive hydrogel could promote the adhesion and proliferation of NIH3T3 cells.
出处 《郑州大学学报(医学版)》 CAS 北大核心 2014年第5期648-650,共3页 Journal of Zhengzhou University(Medical Sciences)
基金 唐山市科技计划项目13130283z 河北联合大学科学研究基金项目z201144
关键词 羧甲基壳聚糖 温敏凝胶 NIH3T3细胞 增殖 牙周组织再生 carboxymethyl chitosan thermosensitive hydrogel NIH3T3 cell proliferation periodontal tissue regeneration
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