链霉素抗性突变理性筛选链霉素高产菌株

Streptomycin-resistant Screening of High Streptomycin-producing Strain

以灰色链霉菌(Streptomyces griseus)CICC11002为出发菌株,采用紫外诱变并结合链霉素抗性筛选法育种,以期获得高产菌株。灰色链霉菌孢子悬液经90%紫外致死剂量诱变后,在含有链霉素最小抑制浓度(0.8μg/m L)的培养基平板上,分离得到105株链霉素抗性突变菌。其中链霉素产量高于出发菌株的有19株,正突变率高达18.1%,同时获得了产链霉素能力约为出发菌株2倍的突变株SM-55。

Streptomyces griseus CICC11002 as the initial strain was treated by UV and screened for Streptomycin resistance in order to improve streptomycin production.The spore suspension was treated with UV light with the dose of 90％ lethal and then inoculated on agar plates containing 0.8 μg/mL Streptomycin for screening.Among one hundred and five Streptomycin-resistant mutants,nineteen mutants had higher Streptomycin yields than the original strain.The positive mutation frequency was up to 18.1％.Finally,mutant SM-55 was obtained.The yield of Streptomycin by this mutant was increased by 199％ compared with that by the original strain.