摘要
目的确定大别山野葛根异黄酮的超声辅助最佳提取工艺与还原力。方法在单因素实验的基础上,进行4因素5水平的Box-Behnken中心组合实验设计,利用响应面分析优化工艺条件,并测定优化工艺条件下葛根异黄酮提取液的还原力。结果大别山野葛根异黄酮300 W功率的超声辅助提取最佳工艺条件为乙醇75%、液料比35 m L·g-1、处理时间35 min、处理温度50℃,此工艺条件下葛根异黄酮得率为24.18%,相对误差仅3.02%;葛根异黄酮还原力的EC50值为0.135 mg·m L-1。结论响应面法适用于对葛根异黄酮超声辅助提取得率进行回归分析和参数优化。
OBJECTIVE To optimize the uhrasonic-assisted extraction process of isoflavones from Pueraria Iobata ( Wild. ) Ohwi using response surface methodology and measure the reducing power. METHODS On the basis of single factor experiments, a four factors and five levels Box-Behnken experiment was designed. Then the reducing power of isoflavones was measured. RESULTS The optimum conditions for the ultrasonic-assisted extraction process of isoflavone from Pueraria Iobata (Wild.) Ohwi at ultrasonic power of 300 W were as follows: ethanol concentration 75 %, liquid-solid ratio 35 mL g-1, extraction time 35 min, and treatment temperature 50 ℃. Under the optimum conditions, the yield of polysaccharide from Pueraria lobata(Wild. ) Ohwi was 24. 18%, and the relative error between experimental value and predicted value was only 3.02% ; the EC50 of the reducing power of isoflavones reached 0. 135 mg mL-1. CONCLUSION The response surface methodology is suitable for regression analysis and optimization of the ultrasonicassisted extraction of polysaccharide from Pueraria lobata ( Wild. ) Ohwi.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第1期51-57,共7页
Chinese Pharmaceutical Journal
基金
2014年安徽省高校优秀青年人才支持计划
安徽省高等学校省级自然科学研究重点项目(KJ2012A280)
国家级大学生创新创业训练计划项目(201310376002)
关键词
葛根
异黄酮
响应面
超声辅助法
工艺
还原力
Pueraria lobata( Wild. ) Ohwi
isoflavone
response surface methodology
ultrasonic-assisted
extraction process
reducing power