免疫金标法与化学法检测胃液隐血方法学评价

Evaluation on gold immunochromatography assay and chemical method in detection of occult blood in gastric juice

目的:浅析免疫金标法(gold immunochromatography assay,GICA)与化学法检测胃液隐血的方法学评价。方法:以我院2012年1月至2013年1月胃液隐血检测的311例患者为研究对象,对其送检的胃液或呕吐物同时行GICA和化学法检查。结果:GICA、化学法的阳性预测率、阴性预测率、诊断符合率分别为64.73%、100.00%、79.74%,70.00%、53.09%、65.59%;两者联合诊断时诊断符合率可达95.38%。结论:GICA的阳性预测值与化学法并无统计学差异,但阴性预测值、诊断符合率明显优于化学法。可通过两法联合诊断来提高检测结果准确性。

Objective：To explore the value of gold immunochromatography assay（GICA） and chemical method in the detection of oc- cult blood in gastric juice. Methods：Totally 311 patients were taken as test samples. Chemical method and GICA were used to detect the gastric juice and vomits. Results：The positive predictive rates of GICA and chemical method were 64.73% and 100%;the negative predicative rates of GICA and chemical method were 79.74% and 70% ;diagnostic accordance rates of GICA and chemical methodwere 53.09% and 65.59% .The combined diagnosis coinci- dence rate can reach 95.38%. The specificity and coincidence of GICA was better than those of chemical method with statis- tically significant differences （P〈0.05）. Conclusion： GICA is su-perior to chemical method in negative predictive value and diagnostic accordance. The combination of both methods can improve the accuracy of the diagnostic therapy