摘要
目的观察丹红注射液对氧化型低密度脂蛋白(ox-LDL)诱导的THP-1巨噬细胞脂质蓄积的影响,并探讨其机制。方法 160 nmol/L PMA孵育THP-1巨噬细胞24 h后,细胞分为三组:对照组、ox-LDL组(100 mg/L)和丹红组(100 mg/L ox-LDL+30 mL/L丹红注射液)。高效液相色谱分析法检测细胞内胆固醇水平,采用液体闪烁计数法观察细胞内胆固醇的流出,Western blot检测细胞ATP结合盒转运体A1(ABCA1)和肝X受体α(LXRα)的蛋白表达,定量PCR检测细胞ABCA1和LXRα的mRNA表达。结果丹红注射液抑制ox-LDL诱导的THP-1巨噬细胞脂质蓄积,显著降低细胞总胆固醇(TC)、游离胆固醇(FC)和胆固醇酯(CE)的含量,增加载脂蛋白AⅠ(ApoAⅠ)介导的胆固醇流出,促进THP-1巨噬细胞ABCA1和LXRα的表达。结论丹红注射液抑制ox-LDL诱导的THP-1巨噬细胞脂质蓄积,可能与其促进LXRα-ABCA1途径介导的胆固醇流出有关。
Aim To explore the effects and mechanisms involved of Danhong injection on lipid accumulation in THP-1 macrophages induced by oxidized low density lipoprotein(ox-LDL). Methods The human THP-1 macrophages were induced by phorbol-12-myristate acetate(PMA,160 nmol /L) for 24 h,then cells were divided into three groups:control group,ox-LDL group(100 mg /L) and Danhong group(100 mg /L ox-LDL + 30 mL /L Danhong injection).Cellular cholesterol was examined by HPLC. Cholesterol efflux was determined by liquid scintillation counting. The protein and mRNA expression of ATP-binding cassette transporter A-1(ABCA1) and liver X receptor α(LXRα) were detected by Western blot and RT-PCR,respectively. Results Danhong injection inhibited the lipid accumulation in THP-1macrophage induced by ox-LDL. Cellular total cholesterol,free cholesterol and cholesterol ester were decreased by application of Danhong injection. Danhong injection increased apolipoprotein AⅠ(ApoAⅠ) mediated cholesterol efflux. In addition,Danhong injection significantly decreased the expression of ABCA1 and LXRα in both protein and mRNA levels.Conclusions Danhong injection inhibits lipid accumulation induced by ox-LDL,and the mechanisms may be related to the activation of LXRα-ABCA1 pathway and the increase of ApoAⅠ mediated cholesterol efflux in THP-1 macrophage.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第7期690-694,共5页
Chinese Journal of Arteriosclerosis