南方红豆杉细胞培养物的化学成分研究

Chemical constituents of Taxus chinensis var. mairei cell cultures

采用硅胶柱色谱、Sephadex LH-20、HPLC等柱色谱技术,从南方红豆杉细胞培养物的80%乙醇(体积分数)提取物中分离得到13个化合物,根据理化性质与波谱数据鉴定化合物的结构分别为:2α,4α,7β,9α,10β-pentaacetoxy-14β-hydroxytax-11-ene(1)、2α,4α,7β,9α,10β-pentaacetoxytax-11-ene(2)、1β-deoxybaccatin VI(3)、2α-acetoxytaxusin(4)、taxuyunnanine C(5)、yunnanxane(6)、2α,5α,10β-triacetoxy-14β-propionyloxy-4(20),11-taxadiene(7)、2α,5α,10β-triacetoxy-14β-isobutyryloxy-4(20),11-taxadiene(8)、2α,5α,10β-triacetoxy-14β-(2'-methyl)butyryloxy-4(20),11-taxadiene(9)、13-dehydroxylbaccatin III(10)、13-dehydroxy-10-deacetylbaccatin III(11)、paclitaxel(12)及β-谷甾醇(13)。其中,化合物1为新化合物,化合物2、4、10和11为首次从南方红豆杉的细胞培养物中分离得到。

The chemical constituents of Taxus chinensis var. mairei cell cultures were investigated by chromatographic methods, including silica gel column chromatography, Sephadex LH-20 and preparative HPLC. Thirteen compounds were isolated from the 80% ethanol extract of cultured cells and their structures were elucidated by spectral data and physicochemical properties, which were identified as 2 a, 4a, 7fl, 9a, 100ft- pentaacetoxy- 14β-hydroxytax- 11-ene （1）, 2a, 4a, 7β, 9a, 10β-pentaacetoxytax- 11-ene （2）, 1β-deoxybaccatin VI （3）, 2a-acetoxytaxusin （4）, taxuyunnanine C （5）, yunnanxane （6）, 2a, 5a, 10fl-triacetoxy-14β-propionyloxy-4 （20）, 11-taxadiene （7）, 2a, 5a, 10β-triacetoxy-14β-isobutyryloxy-4 （20）, 11-taxadiene （8）, 2a, 5a, 10β-triacetoxy-14β- （2＇-methyl）butyryloxy-4 （20）, 11-taxadiene （9）, 13-dehydroxylbaccatin II1 （10）, 13-dehydroxy- 10-deacetylbaccatin III （11）, paclitaxel （12） and （13） β-sitosterol. Among them, compound 1 is a new compound, and compounds 2, 4, 10 and 11 are isolated from the cell culture of Taxus chinensis var. mairei for the first time.