宁神温胆汤对精神分裂症大鼠海马凋亡蛋白Fas、FasL表达的影响

Influence of Ningsheng Wendantang Decoction on Expression of Fas and FasL in Hippocampus of Schizophrenic Rats

目的：探讨宁神温胆汤对精神分裂症大鼠海马凋亡蛋白Fas、Fas L的表达影响。方法：随机将40只SD大鼠分为4组：正常组、模型组、宁神温胆汤组、氯丙嗪组。正常组、模型组给予生理盐水,宁神温胆汤组给予中药药液,15.2 g生药·kg^-11次/d,共21 d;氯丙嗪组先连续灌胃等容量生理盐水14 d,1次/d,最后7 d腹腔注射盐酸氯丙嗪4.5 mg·kg^-1。各组最后一天给药1 h后,正常组腹腔注射生理盐水2 mg·kg^-1外,其它3组腹腔注射阿扑吗啡2 mg·kg^-1,观察15 min大鼠刻板行为,然后断头处死,免疫组化检测Fas、Fas L。结果：与模型组相比,氯丙嗪组在5、10、15 min刻板行为明显减少,宁神温胆汤组在10、15 min刻板行为出现减弱,差异有统计学意义（P〈0.01）;宁神温胆汤组、氯丙嗪组Fas、Fas L表达降低,差异具有统计学意义（P〈0.01-0.05）。结论：宁神温胆汤组有一定的抗精神分裂症作用,可能通过对抗外源性刺激导致的细胞凋亡发挥其神经保护作用。

Objective： To explore the influence of Ningshen Wendan Decoction on apoptosis expressions of Fas and Fas L in hippocampus of schizophrenia rats. Methods： 40 SD rats were randomly divided into normal group（ treated with normal saline）,model group（ treated with normal saline）,Ningshen Wendan Decoction group [a gavage of decoction 15.2 g /（ kg·d）,21 d] and chlorpromazine group [treated with normal saline for 14 d and intraperitoneal injection with chlorpromazine 4. 5 mg /（ kg·d） ]. Each group,except the normal group（ saline）,was intraperitoneally injected morphine 2 mg / kg one hour after administered drugs on the last day. We observed the stereotyped behavior of rats for 15 min and detected the Fas and Fas LResults： Compared with model group,stereotyped behavior in chlorpromazine group decreased at 5,10 and 15 min and Ningshen Wendan Decoction group weakened significantly at 10 and 15 min（ P〈0. 01）.Expressions of Fas and Fas L in chlorpromazine and Ningshen Wendan Decoction groups decreased significantly（ P〈0. 05）. Conclusions： Ningshen Wendan Decoction had antipsychotic effect to a certain extent,playing a neuroprotective role possibly through against exogenous stimulation- induced apoptosis.