反复喘息婴幼儿维生素D3水平及与Th1／Th2细胞平衡变化的相关性研究

Correlations between serum levels of 25 - （OH） D3 and changes in Th1/Th2 cell balance in infants with recurrent wheezing

目的 探讨反复喘息婴幼儿血清25-羟维生素D3［25-（OH）D3］水平与Th1/Th2细胞平衡变化之间的关系。方法 分别采用酶联免疫吸附法（ELISA）、双抗体夹心 ELISA（ABC-ELISA）法检测60例反复喘息的婴幼儿及60例健康对照儿童血清中25-（OH）D3、干扰素-γ（IFN-γ）、白细胞介素（IL）-4、IL-13水平，并分析反复喘息的婴幼儿维生素D3水平与Th1/Th2平衡变化的相关性。结果 反复喘息婴幼儿组血清 25-（OH）D3水平明显低于健康对照组［（18.24±5.64）μg/L比（37.85±7.78）μg/L］（t=15.810，P =0.000）；反复喘息婴幼儿组血清IFN-γ水平明显低于健康对照组［（11.20±2.08）ng/L比（20.68 ±3.87）ng/L］（t=16.700，P =0.000）；反复喘息婴幼儿组 IL-4、IL-13水平明显高于健康对照组［IL-4：（28.61 ±6.44）ng/L比（22.14±5.29）ng/L；IL-13：（20.02±4.83）ng/L比（17.72±4.06）ng/L］（t=6.201，P =0.000；t=2.829，P =0.006）；反复喘息婴幼儿组Th1/Th2低于健康对照组（0.41±0.12比1.00±0.36）（t=11.796，P =0.000）；反复喘息婴幼儿组血清 25-（OH）D3水平与Th1/Th2呈负相关（r= -0.649，P =0.000），健康对照组血清25-（OH）D3水平与Th1/Th2无相关性（r= -0.217，P =0.096）。结论 血清25-（OH）D3水平低下可能为婴幼儿反复喘息的高危因素。反复喘息婴幼儿血清25-（OH）D3水平与 Th1/Th2呈负相关，表明婴幼儿反复喘息与变态反应密切相关。

Objective To explore the relationship between levels of serum 25 - （OH） D3 and changes in Thl/ Th2 cell balance in infants with recurrent wheezing. Methods Sixty cases of infants with recurrent wheezing were involved and 60 cases of healthy children were selected as controls. Enzyme linked immunosorbent assay （ ELISA ） was used to detect the levels of serum 25 - （OH） D3 and double - antibody sandwich（ ABC - ELISA） was used to detect the serum levels of interferon- γ（ IFN-γ） , interleukin （IL） -4, IL- 13, and then the relationship between the levels of serunl 25 - （OH） D3 and changes in Th1/Th2 balance in infants with recurrent wheezing were explored. Results Serum 25 - （OH） D3 levels decreased significantly in the infants with recurrent wheezing group compared with those of the healthy control group [ （ 18.24 ± 5.64） μg/L vs （ 37.85 ± 7.78 ） μg/L] （ t = 15. 810, P = 0. 000 ）. Serum IFN - levels decreased significantly in the infants with recurrent wheezing group compared with those of the healthy control group[ （11.20 ±2.08） μg/L vs （20.68 ±3.87） μg/L] （t = 16. 700,P =0. 000）. In contrast,serum IL -4,IL - 13 levels increased significantly in the infants with recurrent wheezing group compared with those of the healthy control group[IL-4：（28.61 ±6.44） μg/L vs （22. 14±5. 29） μg/L;IL- 13： （20. 02 ±4. 83） μg/L vs （17. 72 ±4.06） μg/L] （ t = 6. 201, P = 0. 0130 ; t = 2. 829, P = 0. 006 ）. Th1/Th2 in the infants with recurrent wheezing group were lower than that those of the healthy control group, and there was statistically significant difference between two groups （0.41 ± 0.12 vs 1.00 ± 0.36） （ t = 11. 796, P = 0.000 ）. Serum 25 - （OH） D3 levels were negatively correlated with Th1/Th2 in the infants with recurrent wheezing （r = -0. 649, P = 0. 000）. There were no correlation between sermn 25 - （OH） D3 levels and Th1/Th2 in the healthy control group （ r = - 0. 217, P = 0. 096）. Conclusions Low serum 25 - （OH） D3 may be the risk factor for recurrent wheezing in infants. Serum 25 - （OH） D3 levels were negatively correlated with Th1/Th2 in the infants with recurrent wheezing group,which show that recurrent wheezing in the infants is closely related to allergic reaction.