纤维素分解微生物复合菌剂降解固态物料特性研究

Characteristics study in the solid material decomposing by cellulose producing microbial compound inoculants

为提高纤维素类碳源物质降解能力,通过试验驯化得到可降解玉米秸秆微生物复合菌剂。将复合菌剂添加到以纤维素类物质(滤纸、打印纸、秸秆、报纸)作为培养基碳源物质的液体培养基中,分析菌剂反应前后培养基中碳源物质减重率。结果表明,滤纸、打印纸、秸秆、报纸减重率分别达到93.7%、83.2%、75.4%、61.3%。玉米秸秆横截面扫描电镜观察表明,复合菌剂处理前后,细胞结构变得疏松,产生裂缝和空洞,有效证实驯化微生物复合菌剂有助于降解纤维素碳源物质能力。为分离复合菌剂中的单菌株,利用功能菌培养机上产生的透明圈及颜色变化,分离出降解蛋白质、脂肪、纤维素及产酸菌株,利用16S r RNA序列分析,鉴定出4个菌属8种菌株,分别为Chryseobacterium indologenes Dan121、Chryseobacterium indologenes Dan191、Acinetobacter bereziniae Zhi71、Acinetobacter bereziniae Zhi77、Acinetobacter bereziniae Zhi111、Lactococcus garviae S36、Bacillus cereus M7、Bacillus cereus MP310。

In order to rise up the gas production during the anaerobic fermentation of agricultural waste, a set of microbial inoculum （CPB） which can degrade maize straw was build up. Cel ulose-riched ma-terials filter paper, copy paper, rice straw and news paper were fermentated in the culture of CPB as the sole carbon source and their weight loss rate were reached at 93.7%, 83.2%, 75.4%and 61.3%, respectively af-ter reaction. Cel ulose-riched materials amended with the CPB were soften into liquid compared to non-amended control. Scanning electron microscopy （SEM） observation showed that the composite system of dealing with the straw cel ulose structure was loose, cracks and holes. Degradation of cel ulose-riched ma-terials may be correlated with the lytic ability of microbial inoculum. Using functional bacteria on the culture of transparent circle and color changes, isolated from compound bacterium agent in the degradation of protein, fat, cel ulose and producing acid strains, secondly using 16S rRNA sequence analysis, respectively identified Chryseobacterium indologenes Dan121, Chryseobacterium indologenes Dan191, Acinetobacter bereziniae Zhi71, Acinetobacter bereziniae Zhi77, Acinetobacter bereziniae Zhi111, Lactococcus garviae S36, Bacil us cereus M7, Bacil us cereus MP310 with four kinds of bacteria of the geus eight strains.