YKL-40在变应性鼻炎患者血清中的表达及与IL-4和IFN-γ相关性分析

Expression of serum YKL-40 in anaphylactic rhinitis patients and association of serum YKL-40 with serum IL-4 and IFN-γ

目的:比较不同类型变应性鼻炎(AR)患者血清中YKL-40的表达水平,分析其与IL-4、IFN-γ的相关性,探讨其在AR发病机制中的可能作用。方法:选取轻度间歇性、轻度持续性、中-重度间歇性、中-重度持续性AR患者各20例作为实验组,正常健康者20例作为对照组。应用酶联免疫吸附法检测各组血清中YKL-40、IL-4、IFN-γ的表达水平。对以上各组数据进行统计学分析。结果:1不同实验组与对照组血清YKL-40相比表达均明显升高,差异均有统计学意义(P<0.05)。轻度持续组与轻度间歇组血清YKL-40相比表达升高,差异有统计学意义(P<0.05)。中-重度持续组与中-重度间歇组血清YKL-40相比表达升高,差异有统计学意义(P<0.05)。在轻度间歇组与中-重度间歇组、轻度持续组与中-重度持续组之间表达差异均无统计学意义(P>0.05)。2不同实验组与对照组血清IL-4相比表达均明显升高,差异均有统计学意义(P<0.05)。各实验组之间血清IL-4表达差异均无统计学意义(P>0.05)。3不同实验组与对照组血清IFN-γ相比表达均明显降低,差异均有统计学意义(P<0.05)。各实验组之间血清IFN-γ表达差异均无统计学意义(P>0.05)。4AR血清YKL-40水平升高与血清IL-4水平呈正相关,与血清IFN-γ呈负相关。结论:1AR血清中YKL-40表达升高,且与IL-4水平呈正相关,与IFN-γ水平呈负相关,提示YKL-40可能通过调节Th细胞的分化,促进Th1向Th2的免疫偏移,参与AR的发病。2与间歇性AR患者相比,持续性AR患者血清中YKL-40表达升高,提示YKL-40可能会促进AR症状的持续发作。

Objective：This study intends to preliminarily discuss the expression and the clinical meaning of se- rum YKL 40 in anaphylactic rhinitis by comparing the different expressions of serum YKL-40 in types of anaphy- lactic rhinitis, and to preliminarily discuss possible mechanism of having anaphylactic rhinitis involved with serum YKL-40 by associating it with serum IL-4 and IFN-γ in anaphylactic rhinitis. Method：Firstly, each select 20 people in our hospital who have anaphylaetic rhinitis respectively according to different levels--mild intermittent, mild persistent, moderate-severe interminttent, moderate-severe persistent as the experimental groups, while 20 healthy people as the nomal control. Secondly, test the levels of serum YKL-40, IL-4 and IFN-γ respectively in each group by the ELISA method. Thirdly, statistically analyze and discuss the collected data. Result..C）There was a rise in the expression of serum YKL-40 between the experimental groups and the nomal control group, which contained a statistical significance （P〈0.05）. There was a rise in the expression of serum YKL-40 between the mild persistent group and mild intermittent group, which contained a statistical significance （P〈0.05）, as well as between the moderate-severe persistent group and the moderate-severe intermittent group （P〈0.05）, while there was no significant difference in expression of serum YKL-40 between the mild intermittent group and the moder- ate-severe interminttent group, as well as between the mild persistent group and the moderate-severe persistent group （P〉0.05）. （2）There was a rise in the expression of serum IL-4 between the experimental groups and the nomal control group, which contained a statistical significance （P〈0.05）, while there was no significant differ- ence between each experimental group （P〉0.05）. （3）There was a reduction in the expression of serum IL-4 be- tween the experimental groups and the nomal control group, which contained a statistical significance （P（0.05）, while there was no significant difference between each experimental group （P〉0.05）. （4）The corelation between serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative. Conclusion..（1）Allergic rhinitis serum YKL-40 expression was increased. The corelation be tween serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative, suggesting that YKL-40 may regulate the differentiation of Th cells, Promote Thl to Th2 immune shift, through this mechanism involved in the pathogenesis of allergic rhinitis. （2）Compared with patients with intermittent allergic rhinitis, persistent allergic rhinitis serum YKL-40 expression was increased, suggesting that YKL-40 may promote the continuous attacks of the symptoms of allergic rhinitis.