摘要
目的探讨外周血维甲酸相关孤儿受体γt(RORγt)及叉头框P3蛋白(Foxp3)mRNA、血清白细胞介素10(IL-10)、IL-6、IL-17的浓度,在大鼠脊髓缺血再灌注损伤后各时程的演变。方法雄性Wistar大鼠50只,制备大鼠脊髓缺血再灌注损伤模型,据取材时间分为4组(术后1 d、3 d、5 d、7 d与对照组,n=10)。采用实时荧光定量聚合酶链反应(RT-PCR)检测外周血RORγt和Foxp3 mRNA的表达和酶联免疫吸附测定(ELISA)检测血清白细胞介素10(IL-10)、IL-6、IL-17的浓度。结果大鼠脊髓缺血再灌注损伤不同时间组外周血RORγt及Foxp3mRNA表达水平及血清IL-10、IL-6、IL-17与对照组比较,差异具有统计学意义(P<0.05或P<0.01)。结论Thl7/调节性T细胞的失衡与大鼠脊髓缺血再灌注损伤后的发生发展密切相关。
Objective This study aimed to investigate the changes of T-helper 17（ Th17） and regulatory T（ Treg） cells with rat spinal cord ischemia / reperfusion injury by determining the mRNA expression of retinoid-related orphan receptorγt（ RORγt） and forkhead box P3 protein（ Foxp3）,and to observe the correlation with the status of inflammation. Methods Fifty adult Wistar rat（ 380 ~ 420g） were randomly divided into five groups,the control group（ n = 10） and four operation groups（ n =40）. The SCⅡ model was established with a self-made device in the operation group. The segments of ischemia / reperfusion spinal cord were properly taken at interval of 1、3、5 and 7 d after reperfusion. The expression levels of plasma RORγt mRNA and Foxp3 mRNA were detected by real-time quantitative PCR（ qRT-PCR）. IL-10、IL-6 and IL-17 levels in plasma detected by ELISA.Results Expression of RORγt mRNA and Foxp3 mRNA and level of IL-10、IL-6 and IL-17 were significantly increased in the operation group compared with the control group,with statistical differences（ P 0. 05 or P 0. 01）. Conclusion The disequilibrium of Th17 and Treg cells has a close relationship with rat spinal cord ischemia / reperfusion injury.
出处
《中风与神经疾病杂志》
CAS
北大核心
2015年第2期112-114,共3页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金资助项目(No.H0912)
湖南省教育厅资助项目(No.14B157)
