木丹颗粒对痛性糖尿病周围神经病变大鼠kv7.3基因表达的影响

Effects of Mudan Granules on Protein Expression of Potassium Channels Kv7.3 in Painful Diabetes Peripheral Neuropathy Rats

目的：研究木丹颗粒对痛性糖尿病周围神经病变（PDPN）大鼠钾离子通道kv7.3蛋白mRNA表达的影响,探讨木丹颗粒对PDPN的治疗作用。方法：选择雄性Wistar大鼠120只为研究对象,先随机取30只为正常组,其余大鼠腹腔注射STZ（53 mg/kg）制作PDPN模型,4周后,用Vonfrey纤维测双后足机械痛阈,痛阈明显下降为PDPN造模成功。再将90只成模大鼠随机分为模型组,木丹颗粒组,苯妥英钠组。分别于药物治疗后第2、4、8周末,各组随机取8只大鼠的L4-L5背根神经节和脊髓,采用RT-PCR技术法检测其钾离子通道kv7.3mRNA表达的情况。结果：钾离子通道kv7.3与正常组比,模型组mRNA表达在各时间点呈下降的趋势（P〈0.05）,与模型组比,木丹颗粒组和苯妥英钠组mRNA表达在各时间点呈上升的趋势,8周时,两组mRNA表达明显增加（P〈0.05）,而两组比较无显著性差异。结论：木丹颗粒对PDPN有一定的治疗作用,其机制可能与钾离子通道kv7.3mRNA表达呈上调有关。

Objective： To research the influence of Mudan Granules on protein expression of potassium channels kv7. 3in pain diabetes peripheral neuropathy rats and to explore the therapeutic effect of Mudan Granules. Methods： 120 male Wistar rats were for research objects. First,30 of them were selected for normal control group and the rest were injected STZ（ 53 mg / kg） in the abdominal cavity to make PDPN model. After 4 weeks,the rats were tested mechanical pain threshold with double Vonfrey fiber at the double after foots. If the pain threshold significantly reduced,the modes were successful. 90 successful models were randomly divided into model group,Mudan Granules group and phenytoin sodium group. In 2nd,4th and 8th weekend after drug treatment,8 rats were randomly taken out from each group to detect the rat dorsal root ganglion and spinal cord potassium channels kv7. 3 mRNA expression with RT- PCR technique method. Results： Potassium channels kv7. 3 mRNA expression at each time point showed a trend of gradual decline and Mudan Granules and phenytoin sodium groups＇ mRNA expression showed gradual rising trends. At the 8th week,two groups showed increasing most significantly（ P〈 0. 05）. Comparison between the two groups had no significant differences. Conclusion：Mudan Granules on diabetic peripheral neuropathy has certain therapeutic effect and its mechanism may be related to the increased potassium ion channel kv7. 3 mRNA expression.