宫颈癌演变过程中PTEN、survivin基因的表达及其意义

Expressions of PTEN and survivin in the development of cervical cancer and their clinical significances

应用qRT-PCR及Western blot技术检测不同阶段宫颈病变组织中PTEN和survivin的表达状况,探讨其临床意义。结果表明:①PTEN mRNA在宫颈癌、CINⅢ组织中的表达显著低于正常宫颈组织(P<0.01),在CINⅡ及CINⅠ组织中的表达亦低于正常宫颈组织(P<0.05);survivin mRNA在宫颈癌、CINⅢ组织中的表达显著高于正常宫颈组织(P<0.01),在CINⅡ组织中的表达亦高于正常宫颈组织(P<0.05),而CIN I组织中则与正常宫颈无差异(P>0.05)。②PTEN蛋白在宫颈癌、CINⅢ、CINⅡ、CIN I组织中显著低于正常宫颈组织(P<0.01),并随着病变程度的增加而减弱。survivin蛋白在宫颈癌、CINⅢ组织中显著高于正常宫颈组织(P<0.01),在CINⅡ中的表达高于正常宫颈组织(P<0.05),而在CINⅠ组织中则与正常宫颈无差异(P>0.05)。说明PTEN和survivin基因在宫颈从癌前病变向宫颈癌演变过程中发挥重要作用,有望成为宫颈癌分子诊断与分子靶向治疗的新靶点。

The expression of PTEN and survivin in the development of uterine cervical cancer were detected by qRTPCR and Western blot techniques.The results were as follows;（1） The expression of PTEN mRNA in cervical cancer and CIN Ⅲ were significantly higher than that in normal cervical epithelium（P〈0.01）.The expression of PTEN mRNA in CIN Ⅱ and CIN I were higher than that in normal cervical epithelium（P〈0.05）.The expression of survivin mRNA in cervical cancer and CIN Ⅲ were significantly higher than that in normal cervical epithelium（P〈0.01）.The expression of survivin mRNA in CIN Ⅱ was higher than that in normal cervical epithelium（P〈0.05）.（2） The expression of PTEN in cervical cancer,CIN Ⅲ,CIN Ⅱ and CIN I were significantly higher than that in normal cervical epithelium（P〈0.01）,and progressively decreased with the development of cervical epithelial lesions.The expression of survivin in cervical cancer and CIN Ⅲ were significantly higher than that in normal cervical epithelium（P〈0.01）.The expression of survivin in CIN Ⅱ was higher than that in normal cervical epithelium（P〈0.05）.These results suggested that PTEN and survivin might play an important role in the progression from CIN to the cervical cancer.PTEN and survivin would be promising biomarkers for potential molecular diagnosis and therapeutic targets of the cervical cancer.