我国人罕见G9P[6]基因型A组轮状病毒LL51695株VP7、VP4、VP6和NSP4编码基因的分子特征

Characteristics analysis on major encoded genes VP7, VP4, VP6 and NSP4 of human G9P [6] rotavirus LL51695

目的 对存于中国疾病预防控制中心病毒病预防控制所病毒性腹泻室的一例人罕见G9P[6]型A组轮状病毒腹泻标本（LL51695）的主要基因片段进行扩增,确定该毒株的分子特征及其进化方式.方法 采用逆转录聚合酶链反应对LL51695标本的VP7、VP4、VP6及NSP4基因片段进行扩增,并进行序列测定.应用A组轮状病毒自动分型工具（RotaC v2.0）对所测基因进行分型.应用DNAStar和MEGA5.0软件与GenBank上的参考株进行同源性和系统进化分析.结果 确定卢龙猪LL51695为罕见的基因型G9-P[6]-15-E1,四个主要基因片段均为猪来源.结论 该毒株极有可能为猪的轮状病毒直接感染人.

Objective To explore the evolutionary and molecular characteristics of rare RVA genetype G9P[6] （LL51695） isolated in our laboratory.Methods Major genome segments of RVA strain LL51695 was amplified by reverse transcription-polymerase chain reaction （RT-PCR）,and the segments were sequenced.The genome segments of LL51695 was determined by the online RotaC genotyping tool （RotaC v2.0）.The reference sequences of every genome segments of RVA was downloaded from GenBank.Homology and phylogenetic evolutionary analyses were conducted using the MEGA 5.0 and DNAStar software package.Results LL51695 isolated from human in Lulong is a rare genotype constellation：G9-P[6]-I5-E1.Conclusion The VP7,VP4,VP6,NSP4 genes were all porcine origins,the strain may be porcine rotaviruses direct infect human.