摘要
目的建立适应展毛地椒提取物中总黄酮含量的分光光度测定方法。方法以芦丁、芹菜素、木犀草素及槲皮素为对照品,比较直接测定法、盐酸-镁粉、亚硝酸钠-硝酸铝-氢氧化钠及三氯化铝显色法在200~600 nm扫描图谱,分析不同方法对展毛地椒提取物中总黄酮含量测定的适应性。结果直接测定法、盐酸-镁粉显色法中,四种对照品溶液与样品溶液均无共有吸收波长。亚硝酸钠-硝酸铝-氢氧化钠法以芦丁为对照品,检测波长为502 nm。三氯化铝法以芹菜素为对照品,检测波长为340 nm。两种方法方法学考察均符合定量测定要求,可用于测定样品溶液中总黄酮。结论硝酸铝显色法测定总黄酮含量是三氯化铝显色法的4倍左右,含量显著偏高。故选用三氯化铝显色法,其干扰小,专属性好,准确性高,且简便快速,更适用于展毛地椒提物中总黄酮的含量测定。
Objective To establish an ultraviolet spectrophotometry method for determining the content of total fl avonoids in extract of Thymus. Methods Wavelength-scanning spectra were compared between 200 and 600 nm with direct method and coloration methods by Mg–HCl, Na NO2–Al(NO3)3 –Na OH, Al Cl3, respectively. Adaptability was analyzed for different methods. Results Direct method and Mg–HCl coloration method can not apply to determine total fl avonoids in the sample solution, because the reference substances do not have the same absorption wavelength with the extract of Thymus. The methodological evaluation of Na NO2–Al(NO3)3–Na OH and Al Cl3 coloration methods both can meet the requirements of quantitative determination, so the later two methods can be used for determining the content of total fl avonoids. Conclusion The content of total fl avonoids determined by Na NO2–Al(NO3)3 –Na OH was four times more than that of Al Cl3. So Al Cl3 coloration method can be simple, rapid, reliable and reproducible, and can be better used for the determination of total fl avonoids in extract of Thymus.
出处
《现代中药研究与实践》
CAS
2015年第1期25-28,共4页
Research and Practice on Chinese Medicines
基金
北京市"十病十药"研发项目(Z131100002513011)
国家自然科学基金项目(面上项目:81173563)
北京中医药大学复方中药制药创新团队基金项目(2011-CX70-13)
关键词
展毛地椒提取物
总黄酮
分光光度法
含量测定
extract of Thymus
total fl avonoids
ultraviolet spectrophotometry
content determination