小立碗藓对重金属镉胁迫的应答特征

Transcriptome Analysis of Physcomitrella patens Response to Cadmium Stress by Bayesian Network

镉是植物非必需的微量重金属元素,镉胁迫引起植物细胞的代谢紊乱,甚至导致细胞死亡。为了探索苔藓植物对镉胁迫的应答机制,采用高通量测序及生物信息学技术分析了藓类模式植物——小立碗藓(Physcomitrella patens)在镉胁迫下的基因表达特征。结果表明,在镉胁迫下,小立碗藓细胞骨架组织、微管运动、DNA修复系统、端粒维护、配子体形成与有性生殖以及与氮代谢等相关基因的表达具有明显的镉胁迫应答特征,暗示了这些基因可能共同参与小立碗藓对镉胁迫的调控反应。该研究结果为阐明植物对镉胁迫的应答机制提供了新的线索。

Cadmium is a non-essential heavy metal for plant growth. Cadmium stress causes cell metabolism distur- bance or death in plant. Here, we performed transcriptome analysis of Physcomitre/la patens during cadmium stress by RNA-Seq. We revealed a new transcriptional network of cadmium stress in plants. The functions of genes that were upregulated or downregulated under cadmium stress included microtubule-based movement, microtubule-based proc- essing, cytoskeleton organization, DNA replication, DNA metabolic process, telomere maintenance and organization, sexual reproduction, urea metabolic process, and nitrogen cycle metabolic process. These proteins may play roles in P. patens under cadmium stress. Our study provides new information for the further research of the molecular mechanisms of plant adaptation to cadmium stress.