确认我国轮状病毒疫苗株LLR基因型为G10P[15]

Genotype of Rotavirus Vaccine Strain LLR in China is G10P[15]

轮状病毒是世界上引起婴幼儿重症腹泻最常见的病原体。轮状病毒感染引起的腹泻除疫苗外目前尚无特效治疗药物。我国兰州生物制品研究所采用G10型羊轮状病毒LLR疫苗株研制的口服轮状病毒活疫苗罗特威(商品名)已于2001年上市。该疫苗基因型被普遍认为是G10P[12]。为证实该疫苗的基因型,本实验采用RT-PCR方法扩增罗特威疫苗株LLR的VP7与VP4基因并进行序列测定、比对和进化分析,结果显示罗特威疫苗株LLR的VP7基因型为G10、VP4基因型为P[15]。综上罗特威疫苗株LLR基因型为G10P[15]。

Rotavirus is the leading causal agent of severe acute gastroenteritis in children aged5years.A specific pharmacologic agent for the treatment of rotavirus-infected children is lacking.In China,only the Luo Tewei oral vaccine（Lanzhou Institute of Biological Products,Shanghai,China）,which is produced from Lanzhou lamb rotavirus vaccine（LLR）,is available.Studies have hypothesized that the genotype of LLR is G10P[12],To identify the genotype of LLR by reverse transcription-polymerase chain reaction,we showed that the VP7 and VP4genotypes of LLR were G10 and P[15],respectively,based on sequencing,alignment and phylogenetic analyses.In conclusion,we identified the genotype of rotavirus strain LLR to be G10P[15].