摘要
目的:观察荭草花不同制备工艺的样品对犬急性心肌缺血的影响。方法:采用冠状动脉左前降支结扎所致犬急性心肌缺血模型,通过心外膜电图描记、硝基蓝四唑(NBT)组织化学染色,考察不同制备工艺的荭草花样品对冠脉结扎所致麻醉犬心肌缺血程度(ΣST)、缺血范围(NST)、心肌梗死面积以及血清中LDH、CK水平的影响。结果:与模型组比较,荭草花经水提醇沉后正丁醇萃取部位可显著降低犬冠脉结扎所致心肌缺血的程度和损伤范围,降低冠脉结扎犬血清中CK、LDH水平(P<0.05或P<0.01)。结论:荭草花经水提醇沉后正丁醇萃取的样品对犬心肌缺血具有显著的保护作用。
Objective: To observe the effect of Polygonum orientale flower extract with different preparation methods on acute myocardial ischemia in mongrel dogs. Methods: The acute myocardial ischemia model of dog was set up though ligation of anterior descending branch of coronary artery in dogs,then the epicardium electrocardiogram was used to measure the degree of myocardial ischemia and the quantitative histological assay was adopted to determine the area of myocardial ischemia. At the same time,the lactic dehydrogense(LDH) and creatine kinase(CK) levels in serum were analyzed. All of them were used to evaluate the protective effect of Polygonum orientale flower with different preparation methods on acute myocardial ischemia in mongrel dog. Results: Compared with the model group,the sample whose preparation methods was extracted by n-butanol after water extracting and alcohol precipitation showed obvious reduction in the area of myocardial ischemia,with decline in ΣST and NST. Moreover,the LDH and CK levels in serum were decreased(P 〈 0. 05 or P 〈 0. 01). Conclusion: The sample which is extracted by n-butanol after water extracting and alcohol precipitation has a protective effect on injury induced by acute myocardial ischemia in mongrel dog.
出处
《中药材》
CAS
CSCD
北大核心
2015年第1期115-118,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81360664)
贵州省中药现代化科技产业专项基金项目(黔科合重G字[2013]4001)
贵州省高等学校创新能力提升计划(黔教合协同创新字[2013]04)
关键词
荭草花
心肌缺血
保护作用
Polygonum orientale L.flower
Acute myocardial ischemia
Protective effect