EB病毒IgG、IgA、IgM抗体和EB-DNA检测在儿童呼吸道感染性疾病中的诊断价值

Diagnostic values of EB virus Ig G,Ig A,Ig M antibodies and EB- DNA in detecting respiratory infection among children

目的研究EB病毒Ig G、Ig A、Ig M和EB-DNA检测在儿童呼吸道感染性疾病中的诊断价值。方法采用酶联免疫吸附法(ELISA法)检测湖南省人民医院431例呼吸道感染患儿的血清EB病毒抗体,同时用荧光探针PCR法检测患儿咽拭子或血浆EB-DNA;另选该院同期189例无呼吸道感染住院患儿为对照组。分析呼吸道感染患儿不同年龄段EB-DNA和EBVCA三种抗体阳性率差异,并通过ROC曲线分析比较各项目在呼吸道感染性疾病诊断中的效能。结果EB-DNA和Ig G、Ig M两种抗体阳性率在不同年龄段呼吸道感染组患儿间差异均有统计学意义(P<0.05);呼吸道感染组工作特征曲线下面积比较EB-Ig M>EB-DNA>EB-Ig A>EB-Ig G;四项检测指标EB-Ig G、EB-DNA敏感性较好,EB-Ig M特异性较好。结论进行EB-Ig G、EB-Ig A、EB-Ig M三种抗体和EB-DNA检测对呼吸道感染性疾病有一定的诊断价值,不同年龄段的儿童由于免疫系统发育处于不同的发展阶段,对EB病毒的免疫应答存在差异。

Objective To research the diagnostic values of EB virus Ig G,Ig A,Ig M antibodies and EB- DNA in detecting respiratory infection among children. Methods The serum samples of 431 children with respiratory infection from the People＇s Hospital of Human Province were subject to detection of EB virus Ig G,Ig A,Ig M antibodies by ELISA,and their throat swabs and plasma samples were subject to EB- DNA detection by PCR. 189 children hospitalized in the hospital during the same period and without respiratory infection were chosen as the controls. The positive rates of EB- DNA and Ig G,Ig A,Ig M antibodies were determined and compared among the infected children of different ages. The efficacy of the indexes in the diagnosis of respiratory infectious diseases were compared using ROC curve. Results The positive rates of EB- DNA and Ig G,Ig M antibodies varied significantly among different age groups of the children with respiratory tract infection（ P 〈0. 05）. Areas under ROC curves for the four indicators were,EB- Ig M〉 EB- DNA〉 EB- Ig A 〉EB- Ig G. The sensitivity of EB- Ig G and EB- DNA was superior,while the specificity of EB- Ig M was better. Conclusions Detection of EB- Ig G,EB- Ig A,EB- Ig M and EB- DNA has reference value for the diagnosis of respiratory infectious diseases. The immune response to the EB virus varies among the infected children due to the different development stages of the immune system at different ages.