Toll样受体2激动剂对糖尿病肾损害影响的小鼠实验研究

Intervention effects of the Toll- like receptors 2 agonist on mice diabetic kidney damage

目的探讨Toll样受体2激动剂对糖尿病小鼠肾损害的影响。方法链脲佐菌素(STZ)腹腔注射建立小鼠糖尿病模型。将糖尿病小鼠分成两组,糖尿病对照组(DM组)和Toll样受体2激动剂干预组(干预组)。用免疫组化方法检测小鼠肾脏巨噬细胞的表达。比较干预组与糖尿病对照组小鼠的血清肌酐、尿素氮、尿白蛋白排泄率以及肾脏巨噬细胞的表达。结果干预组血清肌酐、尿素氮水平以及肾脏巨噬细胞的表达均较DM组增加(P<0.01),伴随蛋白尿的加重。病理学观察结果发现:在DM组,可以看到肾小球体积增大,肾小球囊腔缩小,系膜区增宽,基底膜增厚,以及肾小管肥大。在干预组,上述变化更为显著。结论 Toll样受体2激动剂加重了糖尿病小鼠蛋白尿、肾小球硬化、肾小管损伤、炎症细胞浸润,证实Toll样受体2激动剂可加重糖尿病小鼠肾损害。

Objective To investigate effects of the Toll- like receptor 2 agonist on diabetic kidney damage. Methods Diabetes was induced by intraperitoneal injection of streptozotocin. Diabetes mice were divided into diabetes control group（ DM group） and Toll like receptor 2 excitomotor intervention group（ intervention group）. Immunohistochemistry was used for macrophage staining. The blood urea nitrogen,serum creatinine,urinary albumin excretion and renal macrophage accumulation were compared between two group. Results There was more significant increase in blood urea nitrogen,serum creatinine level and macrophage infiltration in the kidney of intervention group than those of DM group. Urinary albumin excretion increased significantly in the intervention group. Pathological study found that： the glomerular volume increased glomerular cysts shrink,mesangial widening,basement membrane thickening,and renal tubular hypertrophy in the DM group. In the intervention group,these changes were more significant. Conclusion Toll- like receptor 2 agonist in diabetic mice increased proteinuria,glomerular sclerosis,tubular injury,inflammatory cell infiltration,confirmed that TLR2 agonist aggravated renal injury in diabetic mice.