摘要
目的:研究白鲜皮提取物在体外对白念珠菌生物膜及相关基因的影响。方法:M27-A2测定白鲜皮提取物对白念珠菌的最小抑菌浓度(MIC);XTT法评价白鲜皮提取物对白念珠菌生物膜形成及细胞黏附的影响;实时荧光定量RT-PCR(qRT-PCR)检测白鲜皮提取物作用后,ALS3、HWP1基因表达情况。结果:白鲜皮提取物对白念珠菌的MIC为128μg/m L;对生物膜的SMIC50和SMIC80分别为256μg/m L和512μg/m L;与空白对照组相比,不同浓度白鲜皮提取物(16~512μg/m L)对培养1、2、3、4 h的白念珠菌细胞黏附均有明显抑制作用(P<0.05);qRT-PCR结果显示药物作用后ALS3、HWP1基因表达降低(P<0.05)。结论:白鲜皮提取物对体外白念珠菌生物膜有较明显的抑制作用,其机制可能通过抑制ALS3、HWP1等相关基因的表达,从而抑制Ras/c AMP通路的功能。
Objective :To study the effect of cortex dictamni on Candida albicans biofilm and related gene in vitro. Methods : M27 - A2 was used to determine the minimum inhibitory concentration (MIC) of cortex dictamni against C. albi- cans. XTT assay was performed to determine the effects of cortex dictamni on C. albicans biofilms formation and cell ad- hesion. The real- time fluorescent quantitative RT- PCR (qRT -PCR) was used to determine the difference of ALS3 and HWP1 expression between the before and after cortex dictamni induction group. Resuhs:MIC of cortex dictamni against C. albicans was 128 txg/mL. SMIC50 and SMIC80 of cortex dictamni against C. albicans biofilms were 256 I^g/mL and 512 Ixg/mL. Cortex dictamnifrom 16 I^g/mL to 512 I^g/mL show distinct inhibitive effects on adhesion to C. albicans cul- tured for 1,2,3,4 h ( P 〈 0.05 ) ; Real - time RT - PCR results showed that cortex dictamni treatment resulted in a strik- ing down - regulation of ALS3 and HWP1 (P 〈 0, 05 ). Conclusions : Cortex dictamni was demonstrated to have potent ac- tivity against C. albicans biofilms in vitro. These results indicated that inhibition of Cortex dictamni on biofilm formation possibily depends on inhibiting the expression of ALS3, HWP1 and other related genes, then down - regulating the Ras/ cAMP oathwav function.
出处
《中华中医药学刊》
CAS
北大核心
2015年第5期1089-1092,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81173271)