摘要
目的:研究白鲜皮提取物外用抗炎的药效及药量之优选,为皮肤接触性炎症临床合理用药提供参考。方法:以白鲜皮人体外用量30 g/60 kg为实验参考。用水提醇沉法提取白鲜皮有效成分。选用小鼠耳廓二甲苯致炎的经典模型,并涂抹不同剂量白鲜皮提取物于小鼠肿胀耳廓,通过对左右耳圆片称重计算肿胀度,并经过拟合获得药物量效曲线。结果:二甲苯致炎模型的最佳给药剂量为40μL,最佳致炎时间为0.5 h。白鲜皮提取物用量在11-60 g/kg范围内的肿胀抑制作用显著(P〈0.01)。结论:白鲜皮提取物有较强的抗炎作用,其最佳用量为10.1 g/kg,临床推荐抗炎用量为50.5 g/60 kg。
Objective:The experiment research the best dose and effect of Cortex Dietamni extract in anti-inflammation upon external use, which provide the reference for rational drug using in clinic. Methods : Apply 30 g/60 kg, doses of human external use of Cortex Dictamni, as the baseline reference. Use the method of water solution and alcohol sedimentation to extract the active ingredients of Cortex Dictamni. Using the classical inflammatory model of applying dimethylbenzene on mice pinna, and apply different doses of Cortex Dictamni water extracting solution on mice pinna to resist inflammation. Then get the drug dose-effect curve by weighing the section of mice pinna, and get the drug concentration-response curves by fitting with the computer. Results : The best dose of dimetbylbenzene to trigger inflammation is 40 μ L, the best duration is 0.5h. Using Cortex Dictamni during 11-60 g/kg can be the most effective dose in its inhibition of swelling ( P〈0.01 ). Conclusion : Cortex Dictamni extract has strong anti-inflammatory effect. The best dose is 10.1 g/kg. The proposed dosage in clinic should be 50.5 g/60 kg.
出处
《辽宁中医药大学学报》
CAS
2015年第6期50-53,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
上海中医药大学探索性中医药综合实验项目
关键词
白鲜皮提取物
接触性炎症
抗炎
剂量优选
Cortex Dictamni extract
contact dermatitis
anti-inflammation
best dose