摘要
目的建立HPLC法测定丹芪血宁胶囊中丹参素含量的方法。方法采用高效液相色谱法,使用Wonda Sil C18-WR Analytical（5μm,4.6 mm×250 mm）色谱柱,以甲醇-冰醋酸-水（5∶1∶94）为流动相,流速1.0 ml·min^-1,检测波长281 nm。结果丹参素在10.0～200.0μg·ml^-1范围内线性关系良好,其线性方程为y=14197x-2566（r=0.9999）。丹参素的平均回收率为99.08%,R SD=0.16%（n=6）。结论该方法简便、可靠、准确,可作为该制剂的质量控制方法。
Objective To establish the method for the content determination of Danshensu in Danqi Xuening capsule. Methods HPLC method was adopted. Wonda Sil C18-WR Analytical column（ 5 μm, 4.6 mm ×250 mm） was used with mobile phase consisted of methanol-glacial acetic acid-water（5 ∶1 ∶94） at flow rate of 1.0 ml· min^-1. The detection wave length was set at 281 nm. Results There was a good linear relationship in the concent ration range of 10.0 ～200.0 μg· ml^-1, the linear equation was y =14197x-2556（r=0.9999）. The average recovery was 99.08%, with RSD=0.16%（n =6）. Conclusion The method was simple, reliable and accurate, and can be applied to the quality control of the preparation.
出处
《中国中医药现代远程教育》
2015年第10期140-142,共3页
Chinese Medicine Modern Distance Education of China
