眼镜蛇毒对大鼠下丘脑视上核NOS表达的影响

Effect of Naja naja atra venom on expression of NOS in Supraoptic nucleus of rats

目的观察眼镜蛇毒对大鼠视上核NOS表达的影响。方法将SD大鼠随机分为正常对照组、生理盐水组和眼镜蛇毒组,采用免疫组织化学染色法,观察并比较NOS阳性神经元在各组大鼠下丘脑视上核的分布情况。结果眼镜蛇毒组大鼠视上核NOS阳性神经元比生理盐水组、正常对照组表达明显增强(P<0.01)。结论眼镜蛇毒对大鼠下丘脑视上核NOS的表达有上调作用。

Objective To observe the effects of Naja naja atra venom on the expression of NOS in the SON of rats. Methods SD rats were randomly divided into normal group,saline group and Naja naja atra venom group,immunohistochemical staining,observe and compare the distribution of NOS in the hypothalamus of rats in the groups of supraoptic nucleus. Results The number of NOS positive cells in Supraoptic nucleus of Naja naja atravenom group were higher markedly than that of saline and control groups（P〈0.01）. Conclusion Naja naja atra venom appears to facilitate up-regulation of NOS expression in the SON of rats.