大鼠原发免疫性血小板减少性紫癜热盛模型的建立与评价

Establishment and evaluation of heat sheng model of rat primary immune thrombocytopenic purpura

目的建立同时符合中医＂热盛证＂和原发免疫性血小板减少性紫癜外周血血小板减少的大鼠模型。方法采用SD大鼠背部多点注射20%干酵母菌悬液和腹腔注射1∶4稀释的兔抗SD大鼠血小板血清（APS）建立原发免疫性血小板减少性紫癜热盛大鼠模型。并观察受试大鼠的中医证侯特点、血象、骨髓象及丘脑体温调节中枢5-羟色胺（5-HT）的水平。结果模型组大鼠注射后2～6 h体温和日饮水量显著上升,造模第4天出现足趾紫癜和造模第30天出现肠道黏膜出血（P〈0.05）;外周血象中血小板数显著降低,骨髓象巨核细胞数显著减少（P〈0.05）;大脑体温调节中枢5-HT的水平显著增高（P〈0.05）。结论原发免疫性血小板减少性紫癜热盛大鼠病证结合模型基本体现了＂热盛＂所致原发免疫性血小板减少性紫癜大鼠发生紫癜的病理特点。

Objective To establish a rat model at the same time in accordance with the＂hot sheng syndrome＂of traditional Chinese medicine and primary immune thrombocytopenic purpura of peripheral blood platelet reduction.Methods Using back multi-point injection of 20% dry yeast suspension on SD rats and 1∶ 4 dilution of rabbit anti SD rats platelet serum（APS） by intraperitoneal injection to establish a primary immune thrombocytopenic purpura＂heat sheng＂rat model.And observing rats of TCM syndrome characteristics,hemogram,myelogram and serotonin（5-HT） level of the temperature regulating center in thalamus.Results After injection of 2 h ～ 6 h temperature and daily water of the model group rats increased significantly,toe purper showed in fourth day of modeling and intestinal mucosal bleeding in thirty day of modeling（P〈0.05）; Platelet count in peripheral blood decreased significantly,bone marrow megakaryocyte numberreduced significantly（（P〈0.05）; 5-HT level of the temperature regulating center of brain increased significantly（（P〈0.05）.Conclusions The study of the primary immune thrombocytopenic purpura heat sheng rat model of combination of disease and syndrome reflected basically the pathological characteristics of purpura caused by ＂heat sheng ＂in primary immune thrombocytopenic purpura rat mode.