银杏二萜内酯葡胺注射液对缺糖缺氧损伤的SH-SY5Y细胞保护作用被引量：16

Neuroprotective effects of YXETNZ injection on SH-SY5Y cells against injury induced by oxygen-glucose deprivation

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摘要目的探讨银杏二萜内酯葡胺注射液(YXETNZ)对缺糖缺氧(oxygen-glucose deprivation,OGD)损伤的人神经母细胞瘤细胞(SH-SY5Y)的保护作用及可能的机制。方法 SHSY5Y细胞OGD损伤4 h后,与药物一起复氧1 h,然后测定细胞存活率(CCK-8法)、caspase-3/7酶活力、细胞质中核小体含量;蛋白质免疫印迹检测细胞中p-Akt、p-PKA、p-Bad蛋白量的变化。结果 OGD明显提高SH-SY5Y细胞caspase-3/7酶活力和细胞质中核小体含量,明显降低细胞的存活率。YXETNZ能明显提高OGD损伤的SH-SY5Y细胞的存活率,抑制caspase-3/7酶活性,减少细胞核核小体的释放量,上调p-Akt、p-PKA和p-Bad激酶蛋白量,提高Akt和PKA活性,保护神经细胞。结论 YXETNZ对OGD损伤的SHSY5Y细胞具有明显的保护作用,其保护机制可能与细胞内PI3K/Akt/Bad/caspase-3/7、c AMP/PKA/Bad/caspase-3/7细胞通路的激活有关。 Aim To investigate the protective effects of YXETNZ injection on SH-SY5 Y cells damaged by oxygen-glucose deprivation ( OGD ) , and explore its functional mechanisms. Methods After 4 h of OGD, the cells were treated with 25 mg·L-1 drugs for 1 h. Subsequently, cell viabilities were measured by cell counting kit-8 ( CCK-8 kit ) and cell apoptosis was measured by caspase-3/7 assay kit according to manu-facturer’ s instructions. Furthermore, cell death was also detected by ELISA. The levels of phospho-Akt, phospho-PKA,phospho-Bad were evaluated by western blot. Results Oxygen-glucose deprivation significant-ly decreased the cell viabilities of SH-SY5Y cells, while YXETNZ injection significantly increased cell vi-abilities, phospho-Akt, phospho-PKA and phospho-Bad. Furthermore, YXETNZ injection also reduced the activities of caspase-3/7 and cytoplasmic histone-asso-ciated-DNA-fragments contents. Conclusion Our re-searches demonstrat that YXETNZ injection shows good neuroprotective effects on SH-SY5 Y cells after oxygen-glucose deprivation. The underlying mechanisms may be associated with activation of PI3 K/Akt/Bad/caspase-3/7 , cAMP/PKA/Bad/caspase-3/7 signaling pathway.

作者刘秋许治良周军李娜毕宇安王振中萧伟

机构地区中药制药过程新技术国家重点实验室江苏康缘药业股份有限公司

出处《中国药理学通报》 CAS CSCD 北大核心 2015年第7期994-999,共6页 Chinese Pharmacological Bulletin

基金现代中药创新集群与数字制药技术平台(No2013ZX09402203) 国家工信部重大科技成果转化项目

关键词银杏二萜内酯葡胺注射液缺糖缺氧损伤细胞凋亡 PKA Akt Bad YXETNZ injection oxygen-glucose deprivation apoptosis PKA Akt Bad

分类号 R282.71 [医药卫生—中药学] R284.1 [医药卫生—中药学]

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1王海波,殷淑兰,林静,李怡,李义召,陈立云,韩丹春.鼠脑缺血后再灌注c-fos基因表达及银杏黄酮的影响[J].中华老年心脑血管病杂志,2000,2(5):339-342. 被引量：7
2葛建彬,顾锦华,李梅,吴锋,秦正红.银杏内酯A对小鼠脑缺血/再灌注损伤的保护作用及其抑制NF-κB信号通路下调p53、Caspase-3表达的机制[J].中国药理学通报,2012,28(8):1105-1110. 被引量：32

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