转拟南芥AtCBL9和AtCIPK23基因甘蔗对低钾胁迫的响应研究

Research on Response of Transgenic Sugarcane Carrying At CBL9 and At CIPK23 Genes under Low Potassium Stress

以转At CBL1、At CIPK23基因和非转基因甘蔗品种为材料,研究了转At CBL9和At CIPK23基因甘蔗在低钾胁迫下(100μmol/L)的生长及生理特征。结果表明,在低供钾胁迫60天后,转At CBL9和At CIPK23基因甘蔗株高、干物质积累和根系钾含量高于非转基因甘蔗;甘蔗根系活力上升,转基因甘蔗根系活力上升幅度高于非转基因甘蔗;非转基因甘蔗可溶性糖、丙二醛含量、和SOD酶下降,而2个转基因甘蔗中则表现为上升,或下降幅度降低。在甘蔗中过量表达拟南芥At CBL9和At CIPK23基因有利于甘蔗生长和对低钾的抗性。

The characteristics of dry matter, potassium accumulation and the physiological differences were investigated in the roots among At CBL9 and At CIPK23 transgenic lines and wild type YT00-236 under low potassium stress. The results showed that the transgenic line displayed higher height, dry matter accumulation,and potassium content in roots than that of the wild line under the condition of low potassium supplying. Root system vigor of sugarcane increased in response to low K stress, but the increase ratios of two transgenic lines were higher than that of non-transgenic line. The soluble sugar, MDA content and activities of superoxide dismutase（SOD） in non-transgenic line decreased under low K＋ stress. On contrary, they increased in two transgenic lines. In conclude, ectopinc expression of At CBL9 and At CIPK23 in sugarcane would improve the growth of sugarcane and increased the tolerance to low potassium condition.