深低温冻存对牙周膜干细胞膜片增殖和分化能力的影响

Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheet

目的:研究深低温冻存对牙周膜干细胞膜片增殖和分化能力的影响。方法:将牙周膜干细胞膜片用90%胎牛血清+10%DMSO冻存液冻存3月后复苏,采用甲基噻唑基四唑(MTT)法、von Kossa染色和油红O染色法检测冻存对增殖和分化的影响。结果:冻存牙周膜干细胞膜片与新鲜牙周膜干细胞膜片的增殖率和成骨、成脂分化能力没有明显差异。结论:牙周膜干细胞膜片经深低温保存后可继续保持冻存前原有的增殖能力和多向分化能力。

Objective：To investigate the effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cells （PDLSCs）sheet.Methods：We compared cryopreserved PDLSCs sheet with freshly PDLSCs sheet in terms of cell proliferation rate and multilineage differentiation potential by MTT,von Kossa staining and Oil Red staining.Results：Cryopreserved PDLSCs sheet showed a similar cell proliferation rate and multilineage differentiation potential as fresh PDLSCs sheet.Conclusions：This study demon-strates that cryopreservation does not affect the proliferation and differentiation of PDLSCs sheet,supporting the feasibility of PDLSCs sheet cryopreservation in nitrogen.