灰树花多糖对禽流感灭活疫苗预防效果的影响

Influence of Grifola frondosa polysaccharide on preventive effect of avian influenza inactivated vaccine

将正常的清洁级北京油鸡分组饲喂添加不同百分比灰树花多糖的饲料,分别在10日龄、25日龄和40日龄时注射禽流感H5N1灭活疫苗免疫,并在注射免疫的第2天进行血液中禽流感病毒H5亚型抗体(AIVH5 Ab)水平、巨噬细胞吞噬活性和T淋巴细胞亚群(CD3^+/CD4~/CD8^+)含量的测定,统计饲养过程中的饲料消耗以及油鸡体重的增加量,计算不同分组的生产性能。试验结果表明:灰树花组分能提高AIV-H5 Ab的水平,增强吞噬细胞的活性,增加T淋巴细胞亚群的含量,提高北京油鸡的生产性能,对AIV-H5亚型灭活疫苗的预防效果具有良好的促进作用。

Clean Peking yellow chickens were divided into groups and raised with the feeds containing dif-ferent percentages of Grifola frondosa polysaccharide;they were inoculated with avian influenza virus H5N1 inac-tivated vaccine respectively at ages of 10,25 and 40 days,and their sera’s AIV-H5 Ab levels,macrophages’ activities and T-cell subpopulations（CD3 ＋/CD4 ＋/CD8 ＋）were determined on the next day after inoculations;and the different groups’feed consumptions,body weight gains and production performances were analyzed.The results indicated that the G.frondosa components could raise the AIV-H5 Ab level,enhance the macrophages’ activity,increase the T-cell subpopulations,improve the production performance,and in short,promote the preventive effect of AIV-H5N1 inactivated vaccine.