CD19＋CD5＋B细胞和白细胞介素-10在系统性红斑狼疮患者外周血中的变化及意义

Expression and significance of CD19＋CD5＋B cells and interleukin-10 in patients with systemic lupus erythematosus

目的探讨CD19＋CD5＋B细胞及细胞因子IL-10在SLE患者外周血中的变化及意义。方法选取46例SLE患者及10名健康对照，分别用流式细胞术及ELISA法检测外周血中CDl9＋CD5＋B细胞的数量及血清IL-10的水平，并比较其与SLEDAI评分、ESR、补体之间的相关性。采用单因素方差分析，两两比较用LSD-t检验，以Spearman相关进行相关性分析。结果SLE患者外周血中CD19＋CD5＋B细胞百分率[（5．7±2．1）％]明显低于健康对照组[（19．1±2．9）％]（t=2．431，P=0．005），与SLEDAI评分呈负相关（r=-0．292，P=0．049）；SLE患者血清中IL-10的水平[（18．8±13．5）μg／m1]明显高于健康对照组[（8．3±2．9）μg／m1]（t=3．021，P=0．003），且与SLEDAI评分呈正相关（r=0．322，P=0．029）。结论外周血中CD19＋CD5＋B细胞和IL-10是参与SLE发生发展的重要因素，并与疾病活动度相关。

Objective To access the expression and clinical significance of CD19＋CD5＋B cells and interleukin （IL）-I0 in patients with systemic lupus erythematosus （SLE）. Methods Forty-six SLE patients and ten healthy controls were recruited in the Second Affiliated Hospital of Shanxi Medical University. CD19＋CD5＋ B cells subsets were detected with flow eytometry. IL-10 level in serum were detected with enzyme linked immunosorbent assay （ELISA）. The correlation between the expression of CD 19＋CD5＋ B cells and serum level of IL-10 with ESR, systemic lupus erythematosus disease activity index （SLEDAI） score and complement was analyzed. Pair-wise comparison of means of groups was conducted with one-way ANOVA. Comparison between the two groups was conducted by LSD-t test. Correlations between variables were carried out using Spearman＇s rank correlation test. Results The percentage of CD19＋CD5＋ B cells in peripheral blood of SLE patients [（5.7±2.1 ）%] was significantly lower than those in healthy control group [（19.1±2.9）%]（t=-2.431, P=0.005）, and it had a negative correlation with SLEDAI score （r=-0.292, P=0.049）. The IL-10 serum level of SLE patients [（18.8±13.5 ） pg/ml] was significantly higher than the healthy control group [（8.3±2.9） pg/ml] （t=3.021, P= 0.003）, and it had a positive correlation with SLEDAI score （r=0.322, P=0.029）. Conclusion CD19＋CD5＋B cells and IL-10 may participate in the occurrence and development of SLE. The changes of CD19＋CD5＋B cells and IL-10 in the peripheral blood might contribute to the pathogenesis and correlate with the disease activity of SLE.