PD-L1和EGFR在非小细胞肺癌组织中的表达及相关性分析

Expression of PD-Ll and EGFR in non-small cell lung cancer and its clinical relationships

目的探讨程序性死亡因子配体1（PD-L1）和表皮生长因子受体（EGFR）在非小细胞肺癌（NSCLC）组织中的异常表达及其临床相关性。方法收集2009年6月—2011年7月获取的112例有明确组织学病理诊断的NSCLC组织标本、42例良性病变肺组织标本和40例癌旁的正常肺组织标本,用免疫组织化学方法检测PD-L1和EGFR的表达情况,并对其与临床特征的相关性进行分析。结果在NSCLC组织中,PD-L1和EGFR阳性表达率分别为83.9%（94/112）和55.4%（62/112）;在良性病变肺组织中,其阳性表达率分别为9.5%（4/42）和4.7%（2/42）;在正常肺组织标本中,PD-L1和EGFR均无表达。NSCLC组织PD-L1和EGFR阳性表达率高于良性病变肺组织及正常肺组织标本（P〈0.05）。PD-L1表达与NSCLC的临床分期、淋巴结转移和术后生存期有密切的相关性（χ~2=10.053、5.544,8=1.86,P〈0.05）,但与NSCLC患者的性别、年龄、病理分级、肿瘤大小均无显著相关性（P〉0.05）。EGFR的表达与NSCLC患者的术后生存期有密切的相关性（β=1.92,P〈0.05）,但与患者的性别、年龄、临床分期、病理分级、肿瘤大小和淋巴结转移均无显著相关性（P〉0.05）。PD-L1的表达与EGFR的表达呈显著的正相关关系（r=0.621,P〈0.05）。结论 NSCLC中PD-L1和EGFR的表达与NSCLC的病理变化和预后具有密切的相关性,二者联合检测对非小细胞肺癌淋巴结转移、病理分期及预后的评估有重要的临床意义。

Objective To investigate the abnormal expression of programmed death ligand 1 （ PD-L1 ） and epidermal growth factor receptor （EGFR） in non-small cell lung cancer （NSCLC） and its clinical significance. Methods From June 2009 to July 2011, 112 cases of pathological diagnosed NSCLC tissues, 42 cases of benign lung tissue specimens and 40 cases of tumor adjacent normal lung tissue specimens were collected, using immunohistochemical method to detect PD-L1 and EG- FR, and analyzed the correlation and the clinical features. Results In NSCLC tissues, PD-L1 and EGFR positive expression rate was 83.9% （94/112 ） and 55.4% （62/112） ; in benign lung tissue, the positive expression rate was 9.5 % （4/42） and 4.7% （2/42） ; in normal lung tissues, PD-L1 and EGFR expression was none, NSCLC tissue＇ s positive expression rate was higher than that of benign lung lesions and normal lung tissue specimens （ P 〈 0.05 ）. PD-L1 expression has close correlation with NSCLC＇ s clinical staging, lymph node metastasis and postoperative survival rate （ χ^2 = 10. 053 ,χ^2 = 5. 544, χ3 = 1.86, P 〈 0. 05 ）, but with no significant correlation with NSCLC patient gender, age, pathological grade, tumor size （ P 〉 0.05 ）. EGFR expression has positive correlation with NSCLC postoperative survival rate （ β= 1.92, P 〈 O. 05 ） , but no significant correlation with the patients＇sex, age, clinical stage, pathological grade, tumor size and lymph node metastasis （ P 〉 0.05）. There was a significant positive correlation between the expression of EGFR and PD-LI （ r = 0. 621, P 〈 0.05）. Conclusion PD-L1 and EGFR expression in NSCLC and has close correlation with the pathological changes and prognosis, the combina- tion detection for lymph node metastasis staging in non-small cell lung cancer and prognosis evaluation has important clinical significance.