肺癌组织及其肺癌干细胞中FOXO3的表达及意义

FOXO3 expression in lung tissue and lung cancer stem cells and its implication

目的探讨FOXO3在肺癌组织与肺癌干细胞中的表达特点,为肺癌的诊断和治疗提供新的着手点。方法应用免疫组化、RT-PCR及Western blot法检测肺癌组织中FOXO3的表达,同时应用RT-PCR和Western blot法验证肺癌干细胞中FOXO3mRNA及蛋白的表达,比较其在不同分型肺癌中的阴性率。结果免疫组化结果显示FOXO3在肺癌组织中呈明显低表达,RT-PCR和Western blot检测结果显示肺癌组织及肺癌干细胞中FOXO3 mRNA及蛋白均呈明显低表达,FOXO3蛋白在所有病例中的阴性率为66.7%,且随着癌细胞的分化及转移,其阴性率逐渐升高。结论 FOXO3在肺癌组织及肺癌干细胞中均呈低表达,同时其与肺癌的恶性程度、转移密切相关,或许可成为治疗肺癌的新靶点。

Purpose To detect the expression of FOXO3 in lung cancer specimens and lung cancer stem cell, for diagnosis and treat- ment of lung cancer. Methods Immunohistochemistry, semi-quantitative PCR and Western blot were used to detect FOXO3 protein expression in clinical specimens of lung cancer. PCR and Western blot verified FOXO3 mRNA and protein expression of lung cancer cells. Results Immunohistoehemistry showed that the FOXO3 was significantly lower expressed in lung cancer tissues and the corre- sponding lung cancer stem cells; Detection of FOXO3 mRNA and protein by RT-PCR and Western blot showed both had significantly lower expression ; as progression of the disease, there was a clear decreasing trend, in which negative expression rate of FOXO3 protein accounted for 66.7% in all patients; at the same times, the rate of the expression gradually decreased with loss of differentiation and metastasis of the tumor. Conclusions FOXO3 shows low expression in lung tissue and lung cancer stem cells, while it is closely relat- ed to the degree of malignancy and lung cancer metastasis, which may be a new target for the treatment.