微小核糖核苷酸对急性心肌梗死的早期诊断价值研究

Research Value of mi RNA in Early Diagnosis of Acute Myocardial Infarction

目的:探讨微小核糖核苷酸(miRNA)在急性心肌梗死(AMI)早期诊断中的价值。方法:将发病时间<3h的胸痛患者60例根据其诊断结果分为AMI组(n=30)和非AMI组(n=30),另选择正常对照组(n=30),在入院即刻测定mi R-208a、肌钙蛋白、肌红蛋白、磷酸肌酸心肌同工酶(CK-MB),比较各组生化标记物的变化。在AMI组动态监测miR-208a和肌钙蛋白的波动情况;以实时荧光定量聚合酶链反应测定miR-208a。结果 :入院即刻AMI组miR-208a明显高于对照组(P<0.05),但肌钙蛋白和CK-MB与对照组无明显差异;发病1h时的miR-208a显著高于对照组(P<0.05),肌钙蛋白浓度尚处于正常范围内。miR-208a的峰值出现于发病12h,峰值明显早于肌钙蛋白。结论:miR-208a在早期AMI诊断中具备高特异性和高敏感性。

Objective：To explore the value of miR208a in the early diagnosis of acute myocardial infarction （AMI）. Methods：60 Patients with chest discomfort within 3 hours were included, 30 in the AMI group and 30 in the non-AMI group. Another 30 healthy people were also included as the control group. Plasma miR-208a,cardiac troponin I, myoglobin and CK-MB were determined once the patients arrived and then compared to the basic level in the control group. MiR-208a was detected at series times thereafter in the AMI group. In the study,miR-208a was determined by quantitative RT-PCR. Results：MiR-208a was higher in the AMI group than the control group with the first plasma sample（P〈0.05）. At the first hour after the onset of symptoms miR-208a elevated significantly （P〈0.05）, while cardiac troponin I was normal. The peak level of miR-208a appeared within 12 hours. Conclusions：It is confirmed that the novel biomarker miR-208a had great specificity and sensitivity in the early diagnosis of AMI.