泌乳奶牛铜缺乏与机体氧化应激的关系

Relationship between Cu deficiency and oxidative stress of dairy cows

为了探讨泌乳奶牛Cu缺乏与氧化应激的关系,在某集约化牛场根据血浆铜水平,将泌乳奶牛分为铜缺乏组和铜正常组。对两组牛的抗氧化指标(CP、SOD、T-AOC、GSH-Px)和血浆Cu含量进行分析。结果显示:Cu缺乏组的CP水平显著低于Cu正常组,T-AOC水平显著高于Cu正常组。SOD、GSH-Px水平和正常组无显著差异;皮尔逊相关性分析显示,血浆CP与Cu缺乏呈极显著的正相关;T-AOC与Cu缺乏呈极显著的负相关,SOD、GSH-Px与Cu缺乏无相关性;ROC分析结果显示,CP可作为泌乳奶牛铜缺乏的预警指标,临界值为13.7 U/L。结论:泌乳奶牛血浆中CP、T-AOC与铜缺乏密切相关,CP可作为泌乳奶牛Cu缺乏风险的预警指标。

We explored the correlation between copper（Cu）deficiency and oxidative stress of lactating dairy cows,which were divided into two groups based on serum Cu levels：a Cu deficiency group and a control group.Serum levels of ceruloplasmin（CP）,superoxide dismutase（SOD）,total antioxidant capacity（T-AOC）,glutathione peroxidase（GSH-PX）,and Cu were analysed. The results showed that CP levels in the Cu deficiency group were significantly lower than those in the control group,while T-AOC levels were significantly higher,but there were no significant differences between SOD and GSH-PX levels.Pearson correlation analysis showed that serum CP levels were very significantly positively correlated with Cu deficiency,while serum T-AOC levels were very significantly negatively correlated and SOD and GSH-PX serum levels showed no correlations. ROC analysis showed that CP had diagnostic value for Cu deficiency,with a critical value of 13.7 U/L. Hence,CP and T-AOC levels in lactating cows showed strong correlations to Cu deficiency,indicating that serum CP could be used to predict Cu deficiency.